International Journal of Research in Medical Sciences | April 2019 | Vol 7 | Issue 4 Page 1384 International Journal of Research in Medical Sciences Yadav SM et al. Int J Res Med Sci. 2019 Apr;7(4):1384-1387 www.msjonline.org pISSN 2320-6071 | eISSN 2320-6012 Review Article Ground sections of teeth: histopathological study modality Suresh Motilal Yadav, Rupali Wakode, Sourab Kumar*, Abhishek Jadhav INTRODUCTION Various methods are adopted to study the anatomy of teeth, in which Ground section of teeth is utmost simple and effective technique. Several factors determine how a thin tooth section is grounded and studied including of its shape, thickness and its use. The other method, where microtome used decalcified sections of teeth is prepared with Hematoxylin and eosin staining is also effective. Ground sections of teeth are sections prepared without using any chemical and maintaining its anatomy. 1-5 REVIEW OF LITERATURE Apparatus used for ground section of teeth are • Extracted Teeth treated with formalin for 24hours. • Electrical lathe machine (High and Low speed): Grounding done till 4-5mm thickness (Figure 1,2) • Carborundum stone (Rough, Static, Fine). • Xylene. • DPX. 6,7 Method • Teeth are first soaked in 20% formaldehyde for 24 hours. • Washed in water. • Tooth could be sectioned to any thickness by using Ultra-microtomes with diamond cutting blades. (Buehler Isomet low speed saw with a diamond impregnated blade). • Using various available burs, tooth can be grounded from both sides equally, thus making a thin ground section. Department of Oral Pathology, D Y Patil School of Dentistry, Navi Mumbai, Maharashtra, India Received: 10 January 2019 Accepted: 08 March 2019 *Correspondence: Dr. Sourab Kumar, E-mail: sourab.kumar@dypatil.edu Copyright: © the author(s), publisher and licensee Medip Academy. This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT Ground sections of teeth or bone are considered best solutions for the study of any hard tissues in the study of histopathology. Various studies have been performed like progression of dental caries, enamel studies, dentin studies, cementum annulation studies, bone morphologies, age determinaton studies, etc. It is important study part in any forensic studies where mass disasters occurring as natural phenomenon of cyclonic events, earth quakes, floods and volcanic explosions and airplane accidents, industrial accidents and terror related acts. Forensic dentistry has played a vital role and many medico-legal cases are solved by studying the teeth. Ground sections study is important in any part of research. For a dentist this phenomenon is less understood, and this article brings in limelight various methods and study pattern of ground sections of teeth. With this knowledge an understanding and performing ground sections of teeth becomes a well start for dental graduates. Various other modalities in histopathology are decalcification studies, histopathological staining procedures, special stains procedures, immunohistochemistry. All the histopathological modalities have their own importance and are used appropriately in the study of oral pathology. Keywords: Age, Forensic, Medico-legal, Natural, Study, Teeth DOI: http://dx.doi.org/10.18203/2320-6012.ijrms20191359