Global Animal Science Journal-GASJ 8(2):1-17: 2020; DOI: 10.5281/zenodo.3840540 Alshebly et al 2020; Research Article 1 Corresponding author: Ali, M. Alshebly; Email: alialshebly98@gmail.com; Address: Agricultural Chemistry Department, Faculty of Agriculture, Damietta University, Damietta, Egypt Therapeutic Potential of Methanolic Chicory Leaves Extract Against H2O2-Induced Hepatotoxicity in Rats Nabil A. E. Azzaz 1 , Ramadan, A. Hassan 2 and Ali, M. Alshebly 1 1 Agricultural Chemistry Department, Faculty of Agriculture, Damietta University, Damietta, Egypt. 2 Agricultural Chemistry Department, Faculty of Agriculture, Mansoura University, Mansoura, Egypt. Abstract Liver diseases have become a major global health challenge and may be triggered by several toxic chemicals, which include chemotherapeutic agents, thioacetamide, H2O2, certain antibiotics, excessive alcohol consumption, and pathogenic microbes. Hence, safeguarding a healthy liver is vital for good health and well-being. Chicory (Cichorium intybus) belongs to Asteraceae Family and is considered very important medicinal plant in this direction. The present study deals evaluation methanolic extracts of Chicory plant for a period of 45 days at the following dose levels; 50 and 75 mg/kg body weight/day, orally respectively. The activities were studied by assaying aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), total and direct bilirubin (TB)and (DB), total protein (TP), albumin (Alb), catalase (CAT) and Superoxide dismutases (SOD). The methanolic extract of Cichorium intybus at dose 75 mg/kg body weight/day has the highest effectiveness against H2O2- induced hepatotoxicity. Keywords: Cichorium intybus, methanolic extract, Antioxidant activity, Hepatocytotoxicity Introduction Recently, especially after the appearance of various diseases and the disadvantages of the authentical drugs, human had looked back to the folk medicine, which depends mainly on the medicinal plants. Most of the world scientists tried to make the natural plant products to be replaced the synthetic drugs. (Devappa et al., 2011). Common chicory, (Cichorium intybus) is a perennial herbaceous plant of the dandelion family Asteraceae, usually with bright blue flowers, rarely white or pink. This is also known as blue daisy, blue dandelion, blue sailors, blue weed, bunk, coffee weed, cornflower, Hendibeh, horseweed, ragged sailors, succory, wild bachelor's buttons, and wild endive which is native in Europe, and is now common in North America, China, and Australia, where it has become widely naturalized. (Thorat and Raut, 2018). The liver is a vital organ that plays a central role in transforming and clearing chemicals and is susceptible to the toxicity from these agents.