hematol transfus cell ther. 2 0 1 9; 4 1(1) :25–30 www.rbhh.org Hematology, Transfusion and Cell Therapy Original article Genotyping of Dombrock and Lutheran blood group systems in blood donors from the southwestern region of the state of Paraná, Southern Brazil Ieda Bernadete Volkweis Langer a , Jeane Eliete Laguila Visentainer a , Joana Maira Valentini Zacarias a , Katia Teixeira de Meiroz Grilo b , Paulo Roberto Hatschbach b , Rosane Scotti Zimmermann b,c , Ana Maria Sell a,* a Universidade Estadual de Maringá (UEM), Maringá, PR, Brazil b Centro de Hematologia e Hemoterapia do Paraná (HEMEPAR), Curitiba, PR, Brazil c Hemonúcleo Regional de Francisco Beltrão, Francisco Beltrão, PR, Brazil a r t i c l e i n f o Article history: Received 7 January 2018 Accepted 7 June 2018 Available online 8 July 2018 Keywords: ART4 protein BCAM human protein Genotyping techniques Blood group antigens a b s t r a c t Background: Lutheran and Dombrock are two blood group systems with low immunogenic antigens; they can cause mild-to-moderate transfusion reactions. For both, immunopheno- typing is not performed in the pretransfusion routine in Brazil. In addition, the distribution of their antigenic frequencies is an important marker of ethnicity. Thus, the goal of this study was to carry out the genotyping of the LU*01, LU*02, DO*01 and DO*02 alleles of the Lutheran and Dombrock blood group systems in blood donors from the southwestern region of the state of Paraná, Southern Brazil. Method: Genotyping was performed for 251 blood donors by specific allele-polymerase chain reaction. The genotype and allele frequencies were obtained through direct counting and compared with other Brazilian populations using the chi-square test with Yates correction. Results: The distribution of genotype frequencies for LU were 0.4% for LU*01/LU*01, 6.8% for LU*01/LU*02 and 92.8% for LU*02/LU*02 and for DO, they were 19.9% for DO*01/DO*01, 44.6% for DO*01/DO*02 and 35.5% for DO*02/DO*02. The allele and genotype frequencies of LU and DO were similar to those expected for Caucasians, but the DO*01/DO*01 genotype frequency was different to other Brazilian populations. The rare LU*01/LU*01 genotype was found in a loyal blood donor. Conclusion: The genotyping techniques allowed the evaluation of the LU*01, LU*02, DO*01 and DO*02 alleles in blood donors registered in the Hemotherapy Center of the southwestern region of Paraná, Southern Brazil, and contributed to a genotyped blood donor database. © 2019 Associac ¸˜ ao Brasileira de Hematologia, Hemoterapia e Terapia Celular. Published by Elsevier Editora Ltda. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). ∗ Corresponding author at: Universidade Estadual de Maringá (UEM), Departamento de Análises Clínicas e Biomedicina, Avenida Colombo, 5790, Maringá, PR, CEP 87020-900, Brazil. E-mails: anamsell@gmail.com, amsell@uem.br (A.M. Sell). https://doi.org/10.1016/j.htct.2018.06.001 2531-1379/© 2019 Associac ¸˜ ao Brasileira de Hematologia, Hemoterapia e Terapia Celular. Published by Elsevier Editora Ltda. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).