T092 Contribution of multimodal imaging in traumatic maculopathy S. Elbany 1 , H. El Chehab 1 , M. Thibaud 2 , R. Maelle 3 , E. Agard 1 , S. Verrecchia 1 , L. Kodjikian 2 , C. Dot 1 1 Hopital D'Instruction Des Arm ees Desgenettes, Ophtalmology, Lyon, France, 2 H^ opital de la croix Rousse, Ophtalmology, Lyon, France, 3 Centre Hospitalier de Dole, Ophtalmology, Lyon, France Purpose: Closed globe injuries can cause severe macular damages. Accurate diagnosis is important to inform the patient about prognosis, follow-up the lesions, and promptly treat. Methods: We report 5 cases of different traumatic maculopathies. Results: Case 1: A young man was referred for visual loss (20/50) on his right eye after a public road accident. Fundus examination and Optical Coherence Tomography (OCT) displayed a macular choroidal rupture. Nine months later, OCT-A showed a wound healing process including physiological angiogenesis, complicated few months later by an exsudative lesion. Case 2: a man presented with a visual acuity (VA) limited to hand motion and a central scotoma after a trauma (tensioner). OCT confirmed a full-thickness macular hole. Spontaneous resolution was observed after few months, but VA remained low because of irreversible damage of the photoreceptors and retinal pigment epithelium (RPE). Case 3: a young man reported visual loss (20/32) and paracentral scotoma after a blunt trauma (capsule) to his right eye. OCT showed a foveal cyst with subretinal fluid (threat of macular hole). This aspect disappeared spontaneously but RPE changes persisted. Case 4: a man was referred for an expertise, he presented 3 years ago a facial trauma and complained of blurred vision. OCT displayed a sectoral retinal atrophy. OCT-A showed an hypoperfusion in superfical and deep vascular networks in the territory of the cilioretinal artery matching with the defect of the visual field. Case 5: a 12-year-old boy presented an accidental exposure to laser toy. OCT showed severe alterations of the RPE, leading to a final VA of 20/ 32 in his right eye. Conclusions: Multimodal imaging is useful in traumatic maculopathy for earlier and more accurate diagnosis, better understanding of the physiopathology, helpful for the treatment and the follow-up. T011 Selected pharmacology of PlGF neutralization over anti- VEGF on retinal gliosis and RGC survival assessed in a diabetic mouse model I. Etienne, T. Van Bergen, J. Feyen Thrombogenics NV- BE0881.620.924, Preclinical Research, Leuven, Belgium Purpose: In this study, the effect of placental growth factor (PlGF) inhibition on retinal inflammation, reactive gliosis and neurodegener- ation was investigated by immunohistochemistry in the diabetic streptozotocin (STZ) induced mouse model. Methods: Repeated intravitreal injections of an anti-PlGF antibody (5D11D4; 5.4 lg/eye), aflibercept (40 lg/eye) or vehicle were started 7 weeks after diabetes onset in the STZ mouse model (n = 7–30/group). At 8 weeks after diabetes onset, the retinal inflammation and reactive gliosis was measured on serial 7 lm paraffin sections by the quantifi- cation of F4/80 positive cells and the vimentin positive area, respec- tively. The effect on retinal ganglion cell (RGC) density was investigated by Brn3a immunostaining. Results: Repeated administration of 5D11D4 and aflibercept both significantly reduced the percentage of inflammatory cells with 51% Æ 8% and 59% Æ 4%, respectively (p < 0.001 vs. vehicle). Retinal reactive gliosis was significantly reduced with 5D11D4 treat- ment with 51% Æ 7% (p = 0.003) in contrast to aflibercept treatment in STZ diabetic mice (33% Æ 16%, p = 0.14 vs. vehicle). Anti-PlGF treatment did not alter RGC density, whereas aflibercept injection significantly reduced RGC density with 25% Æ 6% (p = 0.01 vs. vehicle). Conclusions: Repeated administration of 5D11D4 or aflibercept sig- nificantly reduced retinal inflammation in a diabetic STZ mouse model. In contrast to aflibercept treatment, neutralization of PlGF has a positive impact on retinal reactive gliosis and does not affect RGC density. F099 New analysis of the Farnsworth D-15 test B. Evans, J. Barbur, M. Rodriguez-Carmona City- University of London, Applied Vision Research Centre- School of Health Sciences, London, United Kingdom Purpose: The Farnsworth dichotomous D15 test has been adopted and continues to be used widely to determine whether an individual meets the colour specification requirements for employment in a given occupation in spite of known difficulties in linking the results of the test to the severity of colour vision loss. The aim of this study was to investigate the variability of the D15 test and the apparent lack of agreement with other tests. The study also investigated the extent to which normal trichromats and subjects with congenital colour defi- ciency (CCD) can make use of red/green (RG), yellow/blue (YB) and luminance signals to complete the D15 task. Methods: RG and YB thresholds were measured with the Colour Assessment & Diagnosis (CAD) test in 590 subjects (325 deutans, 170 protans and 95 normals). Each subject completed several other tests, including the D15. The spectral radiance of each cap when illuminated with D65 was also measured. These data were then used to model the expected changes in RG, YB and luminance signals in normal trichromats and in subjects with CCD. Results: When no crossings or transpositions are allowed on the D15 test, 94% of normal trichromats and 47% of subjects with CCD pass. 26% of the CCDs that pass have RG thresholds above 10 CAD units (one CAD unit describes the RG colour signal strength for young normal trichromats). The modelling work shows that CCD subjects, in particular, can make use of changes in both luminance and YB signals to complete successfully the D15 task. Conclusions: 47% of colour deficient subjects make no errors on the D15 test. Some of these subjects have severe loss of RG chromatic sensitivity. The large variability in D15 results may be accounted for, at least in part, by the large differences in both luminance and RG and YB colour signals predicted in subjects with CCD. F088 Activation of the phosphoinositide 3-kinase pathway promotes axon regeneration of the optic nerve in vivo R. Evans 1 , C. Pearson 2 , J. Cave 1 , S.S. Deshpande 1 , R. Conceic ß~ ao 1 , J. Fawcett 3 , R. Eva 1 , K. Martin 1 , A.C. Barber 1 1 University of Cambridge, Department of Clinical Neurosciences, Cambridge, United Kingdom, 2 National Institutes of Health, National Heart- Lung and Blood Institute, Bethesda- MD, United States, 3 Institute of Experimental Medicine, Centre of Reconstructive Neuroscience, Prague, Czech Republic Purpose: We aimed to investigate activation of the phosphoinositide 3- kinase (PI3K) pathway and its effect on axon regeneration in vivo. Optic nerve crush (ONC) provides a robust injury model, involving crushing the easily accessible optic nerve. The PI3K pathway converts phosphatidylinositol (3,4)-bis-phosphate (PIP2) lipids to phosphatidyli- nositol (3,4,5)-tris-phosphate (PIP3). Phosphatase and tensin homolog (PTEN) acts as a pathway regulator, returning PIP3 to PIP2. PIP3 activates a number of pathways, including the mTOR pathway, which 37 © 2018 The Authors Acta Ophthalmologica © 2018 Acta Ophthalmologica Scandinavica Foundation Acta Ophthalmologica 2018