048 Citation: Hong Ngoc NT, Huong Binh NT, Hong NV, Thang ND, Huong NT, et al. (2020) In-house validation of a lamp kit for diagnosis of Plasmodium, Plasmodium falciparum and Plasmodium vivax in Vietnam. Glob J Infect Dis Clin Res 6(1): 048-053. DOI: https://doi.org/10.17352/2455-5363.000035 https://dx.doi.org/10.17352/gjidcr DOI: 2455-5363 ISSN: CLINICAL GROUP Introduction Up to day, Malaria is still considered as a public health problem in tropical and subtropical regions. In 2018, there were approximately 228 million estimated cases of malaria identified globally [1]. According to a report of the national malaria prevention program, in 2019 Vietnam had 4,665 malaria parasites detected by cyanoscopy reduced 3.08% compared to 2018. Rate of parasite / 1,000 people was 0.048. The number of malaria cases gradually decreased through 10-year period from 2010 to 2019. In 2019 malaria patients decreased by 89.16% compared to 2010. Malaria parasites concentrated mainly in the Central and Central Highland provinces. The number of malaria parasites may be lower than they actually are, due to the presence of asymptomatic malaria and the low density of parasites below the detection threshold of a microscope; According to some unpublished reports in Vietnam, this rate can reach 30-50% [2]. Currently, there are three main groups of methods applied in the diagnosis of malaria parasites: Giemsa stained blood smear, rapid diagnostic test (based on immune method), Molecular biology techniques like PCR, Real time-PCR [3]. Giemsa-stained smear test is still the Abstract Vietnam announced the elimination of malaria in 25 provinces in 2019 and and advance to eliminate malaria nationwide by 2030. Quick, accurate diagnosis and prompt treatment play a very important role in malaria eradication strategies. In this study, we developed the LAMP technique to detect Plasmodium spp., P. falciparum, P. vivax from different kinds of sample such as whole blood, dried blood spot, etc. The advantages of this method included high specificity, fast detection time, and simple equipment use. Primer set was designed for founding on 18S rRNA gene. A positive reaction was visualised with the naked eye, using the color indicator, Malachite Green. Research Article In-house validation of a lamp kit for diagnosis of Plasmodium, Plasmodium falciparum and Plasmodium vivax in Vietnam Nguyen Thi Hong Ngoc, Nguyen Thi Huong Binh, Nguyen Van Hong, Ngo Duc Thang, Nguyen Thu Huong* and Tran Thanh Duong National Institute of Malariology, Parasitology and Entomology, Hanoi, Vietnam Received: 15 July, 2020 Accepted: 28 July, 2020 Published: 29 July, 2020 *Corresponding author: Nguyen Thu Huong, National Institute of Malariology, Parasitology and Entomology, Hanoi, Vietnam, E-mail: Keywords: Plasmodium; LAMP; Green malachite https://www.peertechz.com gold standard in the confirmed diagnosis of malaria case [4]. This method is widely used, most popular in many countries around the world, with an average detection threshold of about 50 - 100 parasites/μL of blood from all Plasmodium spp, low test costs, performed even in laboratory conditions and field testing [1]. However, this method is difficult to detect cases with low parasite density, the testing staff must trained, must be retrained regularly, especially in areas with low prevalence of malaria or eliminate areas where there is little opportunity for testing [5-7]. Rapid diagnostic tests (RDTs) are also widely used, especially in areas where microscopy sites have not yet been available, but RDTs still have limitations such as low parasite detection threshold, false positive rate, and long positive time [5,7,8]. The common disadvantage of these two methods is the low threshold for detection of malaria parasites [9,10]. Therefore, it is estimated that a large number of people with low-density parasite infection are not detected. According to WHO (2014), people carrying low-density parasites below detection threshold of GSB and RDTs may be responsible for contributing 20-50% of the pathogen to malaria vector and vice versa [10]. Molecular biology techniques based on DNA analysis have proven to be more sensitive and specific than microscopic