Int. J. Pharm. Sci. Rev. Res., 21(2), Jul – Aug 2013; nᵒ 52, 293-296 ISSN 0976 – 044X International Journal of Pharmaceutical Sciences Review and Research Available online at www.globalresearchonline.net 293 Ratnakar Balkrishna Lanjewar * , Shardul Sudhakar Kulkarni 2 , Kailas Vasant Chitalkar 3 , Amit Janak Joshi 4 * Department of Chemistry, Dharampeth M.P. Deo Memorial Science Collage, Nagpur, Maharashtra, India. 2 Department of Chemistry, Guru Nanak Khalsa College Matunga, Mumbai, Maharashtra, India. 3 Gadark Lab Ltd, Vikroli, Mumbai, Maharashtra, India. 4 Shimadzu India pvt. Ltd., Andheri Mumbai, Maharashtra, India. * Corresponding author’s E-mail: rb_lanjewar@rediffmail.com Accepted on: 26-05-2013; Finalized on: 31-07-2013. ABSTRACT The present work describes a simple, precise and accurate HPTLC method for estimation of Indapamide as bulk and in tablet dosage form. Validation was carried out in compliance with International Conference on Harmonization guidelines. It employs aluminium backed silica gel 60 F254 TLC plates, (20 cm × 10 cm, layer thickness 0.2 mm) pre-washed with methanol and mobile phase comprising of Ethyl acetate/Water/25% Ammonia (8: 2: 0.1, v/v/v). The developing solvent was run up to 80 mm in CAM AG chamber previously saturated with 20.0 ml of solvent mixture for 20 min. Densitometric scanning was then performed with CAMAG TLC Scanner-IV equipped with win CATS (version 1.4.6) at λ max 241 nm. This solvent system was found to give compact spots for Indapamide with  value 0.62 ± 0.03. The limit of detection and limit of quantitation were found to be 40ng/spot & 120ng/spot for Indapamide. Linear regression analysis showed good linearity (ݎ2= 0 .9989) with respect to peak area in the concentration range of 150–900 ng per spot. The method was validated for precision, accuracy, specificity, and robustness. The proposed method can also be used for routine quality control to accurately determine Indapamide in bulk and tablet dosage form. Keywords: Densitometric scanning, HPTLC, Indapamide, Method development, Method validation. INTRODUCTION ndapamide ,4-chloro-N-(2-methyl-2,3-dihydroindol-1- yl)- 3-sulfamoyl-benzamide is having marked effect on hypertension, as well as decompensated cardiac failure which is given orally. 1 It is a non-thiazide sulphonamide diuretic. Its molecular formula is C16H16CIN3O3S and it has a molecular weight of 365.84 g/mol3. The drug is official in United St at es Pharmacopoeia (USP). 2 It is a white to off- white crystalline powder that is soluble in methanol, ethanol, acetic acid and ethyl acetate, very slightly soluble in ether, chloroform and benzene and practically insoluble in water. Indapamide belongs to a class of medications called Diuretics. Various techniques have been reported for the determination of Indapamide, including colorimetry, 3,4 fluorimetry, 5,6 electrochemical methods, 7,8 Spectrophotometric method, 9 Four HPLC methods 10-13 and few HPTLC methods. 14,15 It is necessary to develop simple, precise and accurate HPTLC method for estimation of Indapamide as bulk and in tablet dosage form. So we develop this method which can be used for routine quality control to accurately determine Indapamide in bulk and tablet dosage form. Indapamide is a thiazide diuretic drug which is used alone or in combination with other with other antihypertensive drugs. Figure 1: Structure of Indapamide M ATERIALS AND M ETHODS Instruments and Apparatus A CAM AG HPTLC instrument consisting of Linomat V Semi-automatic spotter equipped with a 100 µL syringe, Scanner-IV, Twin-trough developing chambers, and viewing cabinet with dual wavelength UV lamps (CAM AG, Muttenz, Switzerland) were used. HPTLC plates used were of aluminium backed silica gel 60F254 with 0.2 mm thickness, 20 × 20 cm (E. Merck, Darmstadt). Sartorius CP224S analytical balance (Gottingen, Germany) and ultrasonic cleaner (Frontline FS 4, Mumbai, India) were used during the research work. Reagents and M aterials Indapamide pure powder with 99.98% purity was kindly gifted by Dishman pharmaceuticals and chemicals Ltd. All the reagents used during the study were procured from Merck (chemicals) Pvt. Limited, Germany and were of analytical grade. Tablets were purchased from the local pharmacy. HPTLC M ethod Development and Validation of Indapamide in Bulk Drugs and Formulation Form I Research Article