Zofenopril attenuates injury induced by ischemia-reperfusion on rat ovary Raziye Keskin Kurt 1 *, Ays ¸e Citil Dogan 2 *, Murat Dogan 3 , Aynur Albayrak 4 , Sefika Nur Kurt 3 , Furkan Eren 3 , Dilek Benk Silfeler 1 , Atilla Karateke 1 , Ersin Fadillioglu 3 and Tuncay Delibasi 5,6,7 1 Department of Obstetrics and Gynecology, Mustafa Kemal University Medical School, Hatay, 2 Zekai Tahir Burak Women’s Health Training and Research Hospital, 3 Department of Physiology, Hacettepe University Medical School, Departments of 4 Pathology, 5 Endocrinology and Metabolism, 6 Pancreas Islet Research Center, Ankara Diskapi YB Training and Research Hospital, and 7 Department of Endocrinology and Metabolism, Hacettepe University Kastamonu Medical School, Ankara, Turkey Abstract Aim: The aim of the study was to investigate the effectiveness of zofenopril in an experimental model of ovarian torsion in rats with histologic and biochemical assessments. Material and Methods: Experimental procedures were performed on 35 female rats (Wistar albino). Rats were randomly divided into five groups as: sham (sham operated, n = 7); vehicle group 1 (torsion–detorsion, n = 7) with 2 h ischemia and 2 h reperfusion; vehicle group 2 (torsion–detorsion, n = 7) with 2 h ischemia and 5 days’ reperfusion; zofenopril group 1 (torsion–detorsion, n = 7) with 2 h ischemia, 2 h reperfusion and a signal dose of oral 15 mg/kg zofenopril; and zofenopril group 2 (torsion–detorsion, n = 7) with 2 h ischemia, 5 days’ reperfusion and 5 days’ oral 15mg/kg zofenopril. A scoring of histopathologic evaluation was performed on the ovaries according to congestion, bleeding, edema, and cellular degeneration. Biochemical assessments included catalase, tissue malondialdehyde and protein carbonyl. Results: Compared with the vehicle groups, histopathologic scores, tissue malondialdehyde and protein carbonyl levels, which reflect oxidative stress markers, were significantly lower in the zofenopril groups. Furthermore, catalase levels were significantly increased in the zofenopril group. Conclusion: Our study results revealed that zofenopril attenuates injury induced by ischemia-reperfusion on rat ovary. Key words: antioxidant, ischemia-reperfusion injury, ovarian torsion, zofenopril. Introduction Ovarian torsion is an emergency condition and is com- monly seen during the reproductive period with an incidence of 2.7%. 1,2 Ovarian torsion occurs first by obstruction of the venous system. The first problem is caused with entrapment of ovarian vessels within the infundibular ligament. The blood flow of ovarian arter- ies continues and the edema in ovaries causes further increase in pressure over the vessels. Arterial circula- tion is also stopped by pressure in the advanced stage. Ovarian necrosis, infarction and bleeding may occur with ovarian ischemia. The treatment of ovarian torsion during the reproductive period is to provide ovarian Received: June 11 2014. Accepted: November 1 2014. Reprint request to: Dr Raziye Keskin Kurt, Mustafa Kemal Üniversitesi Tıp Fakültesi, Kadın Hastalıkları ve Dog ˘um Anabilim Dalı, 31100 Hatay, Turkey. Email: drraziyekeskinkurt@yahoo.com *R.K.K and A.C.D. contributed equally to this work. doi:10.1111/jog.12658 J. Obstet. Gynaecol. Res. 2014 © 2014 The Authors 1 Journal of Obstetrics and Gynaecology Research © 2014 Japan Society of Obstetrics and Gynecology