Nagalo et al. European Journal of Biomedical and Pharmaceutical Sciences www.ejbps.com │ Vol 9, Issue 5, 2022. │ ISO 9001:2015 Certified Journal │ 328 HEALING ACTIVITY OF A TOTAL AQUEOUS EXTRACT OF SACOGLOTTIS GABONENSIS (BAILLE) URBAN ON INDUCED WOUNDS IN WISTAR RAT Ousmane Nagalo 1 , Mama Kone 2 *, N’Guessan Jean-Baptiste Oussou 3 and Angoué Paul Yapo 4 1 PhD Student of Physiology and Pharmacology, Nangui Abrogoua University, Laboratory of Physiology Pharmacology and Pharmacopoeia, Training and Research Unity of Natural Sciences, Abidjan (Côte d'Ivoire). 2 Senior Lecturer of Physiology and Pharmacology, Nangui Abrogoua University, Laboratory of Physiology Pharmacology and Pharmacopoeia, Training and Research Unity of Natural Sciences, Abidjan (Côte d'Ivoire). 3 Lecturer of Physiology and Pharmacology, Nangui Abrogoua University, Laboratory of Physiology Pharmacology and Pharmacopoeia, Training and Research Unity of Natural Sciences, Abidjan (Côte d'Ivoire). 4 Professor of Animal Physiology and Physiopathology, Nangui Abrogoua University, Laboratory of Physiology Pharmacology and Pharmacopoeia, UFR-SN, Abidjan (Côte d'Ivoire). Article Received on 18/03/2022 Article Revised on 08/04/2022 Article Accepted on 28/04/2022 INTRODUCTION African populations are faced with the emergence or re- emergence of infectious pathologies, the treatment and monitoring of which constitute an additional socio- economic problem for them. [1] At the forefront of these pathologies is Buruli ulcer (BU), a skin infection caused by Mycobacterium ulcerans, present in the environment. [2,3] Africa is the most affected region, particularly West Africa where it is highly endemic with a prevalence of 50%, the majority of cases being children under 15. [4] Côte d'Ivoire is one of the most endemic countries with more than 2,000 new cases per year. [5] In 2007, the National Buruli Ulcer Control Program (PNLUB) counted 25,617 cumulative cases from 1978 to 2006. [6] In addition, the WHO notified nearly 30,000 cases in Côte d'Ivoire in 2008. [7] Antibiotic therapy is used for the treatment of BU regardless of the stage of the disease for 4 to 8 weeks, which results in the healing of early lesions, stabilization of the disease or regression of the lesions allowing less dilapidated surgical SJIF Impact Factor 6.044 Research Article ejbps, 2022, Volume 9, Issue 5, 328-337. European Journal of Biomedical AND Pharmaceutical sciences http://www.ejbps.com ISSN 2349-8870 Volume: 9 Issue: 5 328-337 Year: 2022 *Corresponding Author: Dr. Mama Kone Senior Lecturer of Physiology and Pharmacology, Nangui Abrogoua University, Laboratory of Physiology Pharmacology and Pharmacopoeia, Training and Research Unity of Natural Sciences, Abidjan (Côte d'Ivoire). ABSTRACT Buruli ulcer is a chronic, debilitating infection of the skin and soft tissues caused by Mycobacterium ulcerans. In Côte d'Ivoire, the traditional treatment of this pathology is done orally or cutaneously with Sacoglottis gabonensis. It is on six months of mean depending on the patient condition. The objective of this study is to demonstrate for 35 days the efficacy of the total aqueous extract of Sacoglottis gabonensis stem bark (TAESg) on extensive second - degree induced wounds by burns in Wistar rats. For this purpose, 60 rats were divided into 10 groups of 6 rats/group. Burns are induced on all rats of all groups except group 1. Group 1 received no treatment. Groups 2, 3 and 4 were treated orally and received respectively distilled water, Flukocin® 500 mg at a dose of 14.28 mg/kg bw and TAESg at a dose of 3.5 mg/kg bw. groups 5, 6 and 7 treated by the dermal route, respectively received distilled water, Baneocin® 10 g at a dose of 81.6 mg/kg bw and TAESg at the dose of 5000 mg/kg bw. Batches 8, 9 and 10 treated by the oral route associated with the cutaneous route, respectively received distilled water, Flukocin® 500 mg at a dose of 14.28 mg/kg bw, Baneocin® 10 g at a dose of 81.6 mg/kg bw and TAESg at a dose of 3.5 and 5000 mg/kg bw. Healing activity was assessed macroscopically using scoring methods and planimetrically using percentage shrinkage and healing speed. The results indicated a loss of body weight in rats after induction. After one week of treatment, a highly significant increase in weight by the oral and cutaneous routes and very highly significant by the combination of the two routes, was observed in all of the treated rats with TAESg and the reference substances compared to the treated groups with distilled water. Similarly, macroscopic observation of rat wounds showed a decrease in wound surface area leading to a very highly significant decrease in burn scores and inflammation at the level of the combined pathway. The percentage of shrinkage and the speed of healing experienced a highly significant increase by the oral and cutaneous routes and very highly significant by the combined route. Ultimately, TAESg has healing activity by accelerating the process of wound shrinkage and promoting wound closure through the appearance of epithelial tissues. TAESg has a similar activity to that of Flukocin® and Baneocin®. KEYWORDS: Sacoglottis gabonensis, Wounds, Healing, Rat.