ORIGINAL PAPER Evaluation of Some Biosynthesized Silver Nanoparticles for Biomedical Applications: Hydrogen Peroxide Scavenging, Anticoagulant and Thrombolytic Activities Agbaje Lateef 1,2,3 • Sunday A. Ojo 2,3 • Joseph A. Elegbede 2,3 • Musibau A. Azeez 1,3 • Taofeek A. Yekeen 1,3 • Akeem Akinboro 1,3 Received: 23 October 2016 Ó Springer Science+Business Media New York 2016 Abstract The present study examines the hydrogen peroxide scavenging, antico- agulant and thrombolytic activities of silver nanoparticles (AgNPs) that were biosynthesized using extracts obtained from spider cobweb (CB), pod (KP), seed (KS) and seed shell (KSS) of kolanut (Cola nitida). The nearly spherical shaped AgNPs, with surface plasmon resonance of 431.5–457.5 nm, were polydispersed having sizes of 3–50, 12–80, 8–50, and 5–40 nm for CB, KP, KS and KSS-AgNPs respectively. Hydrogen peroxide scavenging activities of 77–99.8% were obtained using 1–20 lg/ml of AgNPs. The particles prevented the coagulation of blood, and also showed thrombolytic activities of 55.76–89.83%, with KSS-AgNPs having the highest activity. Microscopic examination of the lyzed blood clot supported the thrombolytic activities. On the other hand, silver nitrate solution showed negligible activity of 1.92%, while thrombolysis of 7.55, 8.70, 8.93 and 30.19% were obtained for the extracts of KSS, CB, KS and KP respectively. The results herein presented showed potential biomedical applications of the biosynthesized AgNPs to scavenge free radicals and for the management of blood coagulation disorders and thrombotic diseases. Keywords Biosynthesis Á Silver nanoparticles Á Anticoagulant activity Á Thrombolytic activity Á Blood coagulation disorders & Agbaje Lateef agbaje72@yahoo.com; alateef@lautech.edu.ng 1 Nanotechnology Research Group (NANO?), Ladoke Akintola University of Technology, PMB 4000, Ogbomoso, Nigeria 2 Laboratory of Industrial Microbiology and Nanobiotechnology, Ladoke Akintola University of Technology, PMB 4000, Ogbomoso, Nigeria 3 Department of Pure and Applied Biology, Ladoke Akintola University of Technology, PMB 4000, Ogbomoso, Nigeria 123 J Clust Sci DOI 10.1007/s10876-016-1146-0