Eur Urol Suppl 2008;7(3):217 585 THE EFFECTS OF SILDENAFIL CITRATE ON LIPID PER OXIDATION AND ANTI-OXIDANT ENZYMES IN ERYTHROCYTES OF HEALTHY MEN Perk H. 1 , Armagan A. 1 , Soyupek S. 1 , Hoscan M.B. 2 , Sutcu R. , Ozorak A. 1 1 Suleyman Demirel University, Faculty of Medicine, Dept. of Urology, Isparta, Turkey, 2 Baskent University, Faculty of Medicine, Alanya Research and Practice Centre, Dept. of Urology, Alanya-Antalya, Turkey, Suleyman Demirel University, Faculty of Medicine, Dept. of Biochemistry, Isparta, Turkey Introduction & Objectives: citrate in healthy men on antioxidant enzymes activities (catalase-CAT, glutathione peroxydase-GPX and super oxide dismutase-SOD) in erythrocytes and lipid per oxidation index (malondialdehyde-MDA) in plasma. Material & Methods: citrate or placebo. Plasma MDA levels, erythrocyte SOD, GPX and CAT activities were measured at before Results: c P < 0.01, compared with the corresponding control group MDA (nmol/ml) SOD (U/ gr Hb) GPX (U/ gr Hb) CAT (U/ gr Hb) Hour S P S P S P S P 0 (0.7-1.9) (0.8-2.0) (677-911) (642- 879) (4.8-9.2) (6.5-9.2) (80.7-129.9) (62.2-155.4) 2 (0.8-1.5) (0.9-2.1) (677- 801) (6.7-9.4) (6.9-9.6) (85.9-126.7) 6 (0.8-1.7) (1.1-1.7) c (684- (5.4-9.0) (6.7-9.6) c (97.4-169.1) 24 (0.8-1.6) (0.9-1.7) c (768-1022) 846) (4.6-8.8) (5.4-9.4) (54.9-156.5) (67.9-154.0) Conclusions: by elevating SOD and CAT enzyme activities also induces the demolition of super oxide radicals. It is 586 THRESHOLD GENE TRANSFER WITH HSLO ENHANCES SILDENAFIL-INDUCED ERECTILE RESPONSES IN 2-MONTH STREPTOZOTOCIN (STZ)-DIABETIC RATS Zhao W. 1 , Machingal M. 1 , Turner C. 1 , Davies K. 2 , Melman A. 2 , Andersson K.E. 1 , Christ G.J. 1 1 Wake Forest University, Wake Forest Institute for Regenerative Medicine, Winston Salem, United States of America, 2 Albert Einstein College of Medicine, Dept. of Urology, New York, United States of America Introduction & Objectives: Both K channels and the nitric oxide (NO)/cGMP pathway play a critical patients. Given the recent success of K channel gene transfer in ameliorating the decline in erectile capacity observed in experimentally diabetic rats, the purpose of this study was to investigate the possibility that K channel gene transfer with hSlo (the pore forming alpha subunit of the human maxi-K channel) may augment the cavernous nerve (CN) stimulated intracavernous pressure (ICP) responses in the presence of the PDE-5 inhibitor SIL. Material & Methods: week later, control CN stimulations were obtained at 4 mA (a consistent, reproducible non-erectile stimulus in this animal model) and then repeated on the same animal after the IV injection of SIL (Table). Data were expressed as ICP/blood pressure (BP) and analyzed with a 2-Way repeated measures ANOVA. Results: Increases were observed in ICP/BP after SIL in all groups, but in the presence of hSlo the responses to SIL were enhanced at both doses. Group Treatment ICP/BP Before ICP/BP After DM 0.1 mg/kg SIL (n=10) a 1.0 mg/kg SIL (n=10) a DM+hSlo 0.1 mg/kg SIL (n=10) a,b 1.0 mg/kg SIL (n=10) a,b Conclusions: The data suggests that combination therapy with low-dose gene transfer and orally 587 HUMAN NEURAL CREST STEM CELLS TRANSPLANTED IN RAT PENILE CORPUS CAVERNOSUM FOR REPAIR OF ERECTILE DYSFUNCTION Song Y.S. 1 , Lee H.J. 2 , Yun J.H. 1 , Yang W.J. 1 , Park Y.H. 1 , Park I.H. 2 , Ku J.H. , Kim S.U. 2 1 Soonchunhyang University Hospital, Dept. of Urology, Seoul, South Korea, 2 Gachon University Ghil Hospital, Institute for Regenerative Medicine, Incheon, South Korea, Seoul National University Hospital, Dept. of Urology, Seoul, South Korea Introduction & Objectives: One of the causes of erectile dysfunction is the damaged penile cavernous smooth muscle cells and sinus endothelial cells. To investigate the feasibility of applying neural crest stem cells with multipotent capacity to repair injury in penile cavernosum, HNC10.K10 (K10) immortalized human neural crest stem cell line was transplanted into the penile cavernosum of adult rats. Material & Methods: K10 human neural crest stem cell line was generated via transfection of primary cultured human neural crest stem cell (NCSC) with a retroviral vector encoding v-myc. K10 NCSCs were transplanted into the cavernosum of adult rats. K10 NCSC line was studied by reverse transcription-polymerase chain reaction(RT-PCR) for their phenotypic expression for smooth muscle cells (smooth muscle cell actin, calponin, and desmin) was determined immunohistochemically in penile cavernosum of rats 2 weeks post- transplantation. Results: nerve growth factor receptor (p75 LNGFR ). K10 cells also expressed neuronal markers such as desmin) 2 weeks post-transplantation. Conclusions: Human NCSCs transplanted into the rat penile corpus cavernosum were found for the reconstruction of endothelial cells and smooth muscle cells in the corpus cavernosum in the cell therapy for patients with erectile dysfunction. 588 SCORING CORRESPONDENCE IN OUTCOMES RELATED TO ERECTILE DYSFUNCTION TREATMENT ON A 4-POINT SCALE (SCORE-4) Cappelleri J.C. 1 , Bushmakin A.G. 1 , Symonds T. 2 , Schnetzler G. 1 2 Research, Sandwich, United Kingdom, Introduction & Objectives: To map the relationship between the validated Erection Hardness Score (EHS), which was used as the anchor, and other validated patient-reported outcomes (PROs) for use in men with erectile dysfunction (ED): International Index of Erectile Function (IIEF), Quality of Erection Questionnaire (QEQ), Sexual Experience Questionnaire (SEX-Q), and Self-Esteem And Relationship questionnaire (SEAR). Material & Methods: of the other PROs as a dependent variable was applied to analyze the longitudinal data from randomization to end of the 10-week, double-blind, placebo-controlled phase and the 6-week open label phase. EHS data, which were generated at each sexual encounter (event), were averaged per patient over the same recall period that preceded administration of the other PRO questionnaires. The continuous averaged EHS scores were Results: Intercourse Satisfaction, and Overall Satisfaction) and SEX-Q (Erection, Couples Satisfaction, and Individual Satisfaction), as well as the SEAR total score and SEAR Sexual Relationship domain discriminated on all EHS on the QEQ). Although the model did not impose any functional relationship between PRO score and EHS score, an approximately linear relationship existed between the EHS and all other PROs, which was especially pronounced for those PROs that were more directly related to erectile quality/function. The table below gives intervals on scores of selected PRO outcomes linked to EHS categories. Conclusions: The relationship between discrete EHS categories and PRO scores demonstrates the close correspondence of erectile hardness with erectile function (IIEF-EF), erection quality (QEQ), overall sexual experience (SEX-Q), and ED-related psychosocial factors (SEAR) in men with ED. Table. Mapping of Representative Questionnaire Components Against the EHS Erection Hardness Score (EHS) IIEF Erectile Domain QEQ Total Score SEX-Q Total Score SEAR Total Score 1: Not hard* † 2: Hard but not hard enough for penetration † penetration but not completely hard 18–25 49-79 41–64 4: Completely hard and fully rigid ³26 ³80 ³65 ³74 *Category 1 represents pooled categories 0 (no enlargement) and 1 (larger but not hard). † It was not