1 TOXYCITY OF EXTRACT AND FRACTIONS OF PUSPA BARK (Schima wallichii Korth) TO Artemia salina Leach.* Ade Zuhrotun # , Eli Halimah, Ajeng Diantini, Zeili Prameswari Faculty of Pharmacy, Universitas Padjadjaran #Author for Correspondence: Jl. Raya Sumedang-Bandung KM 21, Jatinangor, West Java, Postal code 45363, Phone/Fax 022-7796200, Mobile Phone 081322517899, email address: airkusaja@yahoo.com Field Natural products-Pharmacology Oral Presentation Puspa (Schima wallichii Korth.) is a medicinal plant which potential to be doveloped as anticancer. Previously research reported that puspa leaves have a cytotoxic activity. In this present works, a toxicity test to extract and fractions of puspa bark was carried out by Brine Shrimp Lethality Test (BSLT). Extraction was done by maceration using ethanol 70%. The concentrated extract was dissolved in water and then fractionated by Liquid-Liquid Extraction (LLE) using n-hexane and ethyl acetate respectively. The result of toxicity test of ethanol extract and fractions of LLE indicated that all sample had a toxic effect to A. salina (LC50 < 1000 ppm). The highest toxycal fraction, that was ethyl acetate fraction (LC50 = 24,70 ppm), then was further separated by classical column chromatoghraphy (CC). The group of fractions II from CC process, showed the highest toxycal activity (LC50 = 11,40 ppm). Preliminary identification of active compound by phytochemical screening and TLC with several spray reagent, predicted that it was terpenoid or polyphenol, however the identity of active compound was still unknown. Keywords: Schima wallichii Korth.. toxicity, Brine Shrimp Lethality Test (BSLT). *This article has been presented in 1 st Pharmaceutical Sciences Conference and Exhibition (PSCE) 2010. 27 & 28 September 2010, Vistana Hotel, Penang. Organized by Pharmacy School Postgraduate Society, Universiti Sains Malaysia