Citation: Koonyosying, P.; Kusirisin, W.; Kusirisin, P.; Kasempitakpong, B.; Sermpanich, N.; Tinpovong, B.; Salee, N.; Pattanapanyasat, K.; Srichairatanakool, S.; Paradee, N. Perilla Fruit Oil-Fortified Soybean Milk Intake Alters Levels of Serum Triglycerides and Antioxidant Status, and Influences Phagocytotic Activity among Healthy Subjects: A Randomized Placebo-Controlled Trial. Nutrients 2022, 14, 1721. https://doi.org/10.3390/ nu14091721 Academic Editors: Felicite Noubissi-Kamdem, Anthony L. Walker and Jean Christopher Chamcheu Received: 17 March 2022 Accepted: 19 April 2022 Published: 21 April 2022 Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affil- iations. Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). nutrients Article Perilla Fruit Oil-Fortified Soybean Milk Intake Alters Levels of Serum Triglycerides and Antioxidant Status, and Influences Phagocytotic Activity among Healthy Subjects: A Randomized Placebo-Controlled Trial Pimpisid Koonyosying 1 , Winthana Kusirisin 2 , Prit Kusirisin 3 , Boonsong Kasempitakpong 2 , Nipon Sermpanich 2 , Bow Tinpovong 4 , Nuttinee Salee 4 , Kovit Pattanapanyasat 5 , Somdet Srichairatanakool 1, * and Narisara Paradee 1, * 1 Oxidative Stress Cluster, Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand; pimpisid.k@cmu.ac.th 2 Department of Family Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand; wkusiris@gmail.com (W.K.); dr.boonsong@gmail.com (B.K.); nipon.s@cmu.ac.th (N.S.) 3 Division of Nephrology, Department of Internal Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand; jingprit@hotmail.com 4 Program of Food Production and Innovation, Faculty of Integrated Science and Technology, Rajamangala University of Technology Lanna, Chiang Mai 50300, Thailand; bowtinpovong@rmutl.ac.th (B.T.); nattinee@rmutl.ac.th (N.S.) 5 Office of Research and Development, Faculty of Medicine and Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand; kovit.pat@mahidol.ac.th * Correspondence: somdet.s@cmu.ac.th (S.S.); narisara.p@cmu.ac.th (N.P.); Tel.: +66-5393-5322 (S.S. & N.P.) Abstract: This study aimed to develop perilla fruit oil (PFO)-fortified soybean milk (PFO-SM), identify its sensory acceptability, and evaluate its health outcomes. Our PFO-SM product was pasteurized, analyzed for its nutritional value, and had its acceptability assessed by an experienced and trained descriptive panel (n = 100) based on a relevant set of sensory attributes. A randomized clinical trial was conducted involving healthy subjects who were assigned to consume deionized water (DI), SM, PFO-SM, or black sesame-soybean milk (BS-SM) (n = 48 each, 180 mL/serving) daily for 30 d. Accordingly, health indices and analyzed blood biomarkers were recorded. Consequently, 1% PFO-SM (1.26 mg ALA rich) was generally associated with very high scores for overall acceptance, color, flavor, odor, taste, texture, and sweetness. We observed that PFO-SM lowered levels of serum triglycerides and erythrocyte reactive oxygen species, but increased phagocytosis and serum antioxidant activity (p < 0.05) when compared to SM and BS-SM. These findings indicate that PFO supplementation in soybean milk could enhance radical-scavenging and phagocytotic abilities in the blood of healthy persons. In this regard, it was determined to be more efficient than black sesame supplementation. We are now better positioned to recommend the consumption of PFO-SM drink for the reduction of many chronic diseases. Randomized clinical trial registration (Reference number 41389) by IRSCTN Registry. Keywords: Perilla frutescens; fruit oil; ω3-PUFA; acceptance; antioxidant; health 1. Introduction The Perilla frutescens var. nga-keemon plant (known by its Japanese name “shiso”) is cultivated in the upper northern provinces of Thailand, specifically Chiang Mai, Nan, and Maehongsorn Provinces. It is an edible fruit that is commonly used as an additive in rice, cookies, and biscuits. P. frutescens oil (PFO) is abundant with omega-3 polyunsaturated fatty acids (ω3-PUFA), particularly α-linolenic acid (ALA) at 54–65% (w/w) of the total fatty acid content [1–3]. In the human body, ALA is metabolized into eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and eicosanoids, all of which are known to exert Nutrients 2022, 14, 1721. https://doi.org/10.3390/nu14091721 https://www.mdpi.com/journal/nutrients