1 3 Adult Opisthorchis felineus major protein fractions deduced 4 from transcripts: Comparison with liver flukes Opisthorchis viverrini 5 and Clonorchis sinensis 6 7 8 Mikhail Pomaznoy a,⇑ Q1 , Sergey Tatkov a , Alexey Katokhin a , Dmitry Afonnikov a,b , Vladimir Babenko a , 9 Dagmara Furman a,b , Ilya Brusentsov a , Pavel Belavin a , Alexandr Najakshin c , Sergey Guselnikov c , 10 Gennady Vasiliev a , Anton Sivkov a , Egor Prokhortchouk d , Konstantin Skryabin d , Viatcheslav Mordvinov a 11 a Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, pr. Lavrentieva 10, Novosibirsk 630090, Russia 12 b Novosibirsk State University, ul. Pirogova 2, Novosibirsk 630090, Russia 13 c Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, pr. Lavrentieva 8, Novosibirsk 630090, Russia 14 d Bioengineering Center, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7/1, Moscow 117312, Russia 15 16 17 19 highlights 20 21  We obtained the first transcriptomic 22 data for Opisthorchis felineus. 23  Sequences were annotated with 24 BLAST and Interproscan algorithms. 25  Trematode specific orthologous 26 clusters were obtained. 27  Phylogenetic analysis of 28 Opisthorchiidae species was 29 undertaken. 30 graphical abstract 32 32 34 article info 35 Article history: 36 Received 11 December 2012 37 Received in revised form 8 July 2013 38 Accepted 11 July 2013 39 Available online xxxx 40 Keywords: 41 Opisthorchis felineus 42 EST 43 Transcriptomics 44 Phylogeny 45 46 abstract 47 The epidemiologically important liver flukes Opisthorchis felineus, Opisthorchis viverrini, and Clonorchis 48 sinensis are of interest to health professionals, epidemiologists, pharmacologists, and molecular biolo- 49 gists. Recently the transcriptomes of the latter two species were intensively investigated. However our 50 knowledge on molecular biology of O. felineus is scarce. We report the first results of the O. felineus tran- 51 scriptome analysis. We isolated and annotated a total of 2560 expressed sequence tag (EST) sequences 52 from adult O. felineus (deposited within the database of expressed sequence tags (dbEST), under accession 53 numbers GenBank: JK624271–JK626790, JK006511–JK006547, JK649790–JK649792). Clustering and 54 analysis resulted in the detection of 267 contigs. Of the protein sequences deduced from these, 82% 55 had homologs in the NCBI (nr) protein database and 63% contained conserved domains, allowing the 56 functions to be interpreted using the Gene Ontology terms. Comprehensive analysis of Opisthorchiidae- 57 and Trematoda-specific substitutions within amino acid sequences deduced for the proteins myoglobin, 58 vitelline precursor protein, cathepsin F, and 28 kDa glutathione transferase was carried out. The gene set 59 of the 32 ribosomal proteins for the three Opisthorchiidae species with the addition of available Schisto- 60 soma and Fasciola orthologs was created and is provided in the supplementary. The orthologous gene set 61 created was used for inferring phylogeny within the Trematoda with special attention to interrelations 0014-4894/$ - see front matter Ó 2013 Published by Elsevier Inc. http://dx.doi.org/10.1016/j.exppara.2013.07.011 ⇑ Corresponding author. E-mail addresses: pomaznoy@bionet.nsc.ru (M. Pomaznoy), tatkov@bionet.nsc.ru (S. Tatkov), katokhin@bionet.nsc.ru (A. Katokhin), ada@bionet.nsc.ru (D. Afonnikov), bob@bionet.nsc.ru (V. Babenko), furman@bionet.nsc.ru (D. Furman), brusentsov_ilja@bionet.nsc.ru (I. Brusentsov), belavin@bionet.nsc.ru (P. Belavin), najakshin@mcb.nsc.ru (A. Najakshin), sguselnikov@mcb.nsc.ru (S. Guselnikov), gnn@bionet.nsc.ru (G. Vasiliev), sivkov@bionet.nsc.ru (A. Sivkov), prokhortchouk@biengi.ac.ru (E. Prokhortchouk), skryabin@biengi.ac.ru (K. Skryabin), mordvin@bionet.nsc.ru (V. Mordvinov). Experimental Parasitology xxx (2013) xxx–xxx Contents lists available at SciVerse ScienceDirect Experimental Parasitology journal homepage: www.elsevier.com/locate/yexpr YEXPR 6718 No. of Pages 10, Model 5G 30 July 2013 Please cite this article in press as: Pomaznoy, M., et al. Adult Opisthorchis felineus major protein fractions deduced from transcripts: Comparison with liver flukes Opisthorchis viverrini and Clonorchis sinensis. Exp. Parasitol. (2013), http://dx.doi.org/10.1016/j.exppara.2013.07.011