Available on line www.jocpr.com Journal of Chemical and Pharmaceutical Research __________________________________________________ ISSN No: 0975-7384 CODEN(USA): JCPRC5 J. Chem. Pharm. Res., 2011, 3(2):907-910 907 Quantitative determination of Yohimbine alkaloid in the different part of the Rauvolfia tetraphylla Arvind Kumar, M. K. Bhardwaj, Upadhyay A. K., Anupa Tiwari and Bikram Deo Ohdar R&D Deptt., Patanjali Ayurved, D-38, Industrial Area, Haridwar (Uttrakhand) India ______________________________________________________________________________ ABSTRACT Rauvolfia belongs to the family Apocynaceae. Rauvolfia tetraphylla is economically important medicinal plant because of the presence of various alkaloids. Alkaloid yohimbine is hypotensive a cardiovascular depressant and hypnotic. In our study we have taken the methanol extract of leaves, stem and roots of Rauvolfia tetraphylla and yohimbine as standard. The compression was done by HPTLC. The quantification and identification of yohimbine was done by WINCATS software with densitometric detection (Camag Scanner -3). The present technique allowed different separation profiles that can be useful for phytochemicals characterization of various parts of plants. The linear calibration ranges were 10-1000 g/ml for yohimbine. Yohimbine was detected only in the leaves of plant in 6.11 %. The HPTLC methods were successfully validated and applied to the quantization of yohimbine. Key words: Alkaloid, HPTLC, Rauvolfia tetraphylla, yohimbine. ______________________________________________________________________________ INTRODUCTION An aphrodisiac is defined as any food or drug that arouses the sexual instinct, induces veneral desire and increases pleasure and performance. Although there are many aphrodisiac in the market, there is no scientific evidence that most of them work. Although Yohimbine has been extensively used by men to enhance sex drive and approved by many food and drug authority effects. Rauvolfia is a genus of evergreen trees and shrubs in the Apocynaceae family. The approximately 85 species in the genus can mainly be found in tropical regions. Rauvolfia