New Biotechnology · Volume 29S · September 2012 recombinant fusion HBsAg scFv protein was tested using ELISA. The present prelimarily finding indicate that anti-HBsAg scFv AP conjugate could be further used to develop a one-step HBV detec- tion system. http://dx.doi.org/10.1016/j.nbt.2012.08.426 Poster 4.3.7 Solid Phase Peptide Radiolabeling: An Efficient Method for the Radio-iodination of the Biomimetic Ligands Vladimír Proks ∗ , Jan Kuˇ cka, Ognen Pop-Georgievski, Jana Svobodová, Tomᡠs Sedlaˇ cík, Hana Studenovská, Eliˇ ska Chánová, Frantiˇ sek Rypᡠcek Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic The iodine radiolabeled bioactive peptides are widely used as efficient tools in tissue engineering 1 , radio imaging 2 and radiotherapy 2 . Current approaches are based on direct radio- iodination of the peptide through chemical modification and purification. In addition to demand of well–equipped radiochem- ical laboratory, large amount of radioactive waste is generated during the radiolabeling, modification and purification steps, which represents main disadvantage. The presented route uses the Chloramine–T radio-iodination reaction as one step in solid-phase peptide synthesis. The main advantage of this approach is, that the orthogonally-protected radio-labeled peptide attached to a solid support allows further incorporation of various reactive functional groups, e.g., alkyne and azide 3 for click chemistry, thiole, maleinimide, lipoic acid for attachment on the gold surface, or (meth)acroyl group for radical polymerization. In addition, the purification step remov- ing the unbound radioactive iodine is very efficient with the peptide bound to the solid support. After deprotection and cleavage the labeled ligand is obtained in high radiochemi- cal yield and purity. The solid-phase approach is presented here on preparation of fibronectin-derived N-terminus-substituted GGRGDSGGGY( 125 I)–NH 2 peptide and biomimetic modification of the various substrates for tissue engineering. Acknowledgement: The work was supported by Grant Agency of CR (No.:P108/11/1875) and ASCR-TUBITAK(111M031). References: Massia, S. P.; Hubbell, J. A. JOURNAL OF BIOLOGICAL CHEM- ISTRY 1992, 267, 14019-14026. Lambrecht, R. M.; Sajjad, M.; Bakr, S. Journal of Nuclear Medicine 1988, 29, 1324-1324. Proks V.;Jaroˇ s J.; Pop-Georgievski O.; Kuˇ cka J.; Popelka ˇ S.; Dvoˇ rák P.; Hampl A.; Rypᡠcek F. MACROMOLECULAR BIO- SCIENCE DOI = 10.1002/mabi.201200095 http://dx.doi.org/10.1016/j.nbt.2012.08.427 Stream: Purple- Systems Biology & Nanotechnology, Session: Nanobiotechnology Poster 4.4.1 Development of a portable bioagent detection and early warning system Hüseyin Avni Öktem ∗ NANObiz Ltd. Co. METU Technopolis Galyum Blok 06800 Ankara, Turkey E-mail address: haoktem@metu.edu.tr. Identification of human pathogens has been an important issue since they had been discovered as the causative agents of infectious diseases. Their potential to be used as weapons, and the recent outbreaks such as SARS, EHEC, swine and bird flu have increased the importance of having a reliable detection and early warning systems for these pathogens. To effectively respond to an event where there is an inten- tional release of a biological agent of unknown origin, first the nature of the biological contamination and the degree of expo- sure must be known. Currently, several detection technologies are available in the market.The most commonly used conventional identification techniques, for example, rely on culturing and fur- ther biochemical analysses to identify biological agents. These methods, although they are accurate, are slow and not applica- ble for non-culturable microorganisms. Current nucleic acid and antibody based approaches, on the other hand, are faster but they need a well-equipped laboratory infrastructure. This study describes the Biological Agent Detection and Early Warning System (BADNEWS). BADNEWS is a field deployable bio-threat agent detection system with autonomous sampling capabilities. BADNEWS relies on a sandwich hybridization based biosensor platform that can be used in both nucleic acid and immuno-based identification of microbial targets. Furthermore, the biosensor platform on which we are still conducting R&D activities has also been integrated into a portable device with com- munication capabilities to be used in the field conditions. http://dx.doi.org/10.1016/j.nbt.2012.08.428 Poster 4.4.2 Development of an Aptamer Based Biosensor for Salmonella Identification Hüseyin Avni Öktem ∗ METU Dep. of Biological Sciences, 06800, Ankara, Turkey E-mail address: haoktem@metu.edu.tr. Food safety is a global health issue since trading of contami- nated food between countries increases the potential for outbreaks. Salmonella is one of the major causes of food borne illness and con- tamination of it also causes economic problems with increased healthcare costs, product recalls and decreased production effi- ciency. Therefore it is very crucial to perform fast, accurate and economical screening and detection tests for Salmonella at each step of the food production chain. S154 www.elsevier.com/locate/nbt