Emir. J. Food Agric. 2010. 22 (5): 406-411 http://ffa.uaeu.ac.ae/ejfa.shtml 406 Short Communication Induction of chlorophyll mutants in Zingiber officinale Roscoe by Gamma Rays and EMS Nilesh Pawar ∗ , Sandeep Pai, Mansingraj Nimbalkar, Firdose Kolar and Ghansham Dixit Department of Botany, Shivaji University, Kolhapur 416 004 (MS), India Abstract: The present investigation comprises the data on frequency percentage of chlorophyll mutations in the different doses of gamma rays coupled with EMS in ginger. Different mutation frequencies and width of mutation spectra were induced under the action of different concentrations of the two applied mutagens. The spectrums of chlorophyll mutations (albino, xantha, and chlorina) were observed and grouped. The overall mutation spectrum for ginger showed that xantha occurred with the highest frequency, followed by chlorina and albino. The mutagenic effectiveness decreased with the increase in dose of mutagen for both the mutagen indicating that negative relationship between effectiveness and dose of mutagen. EMS provided more number of chlorophyll mutants than gamma rays in this investigation. Keywords: Ginger, Chlorophyll mutants, EMS, Gamma rays. اﻟﺰﻧﺠﺒﻴﻞ ﻧﺒﺎت ﻓﻰ اﻟﻜﻠﻮرﻓﻴﻞ ﻟﻤﺎدة اﻟﻄﻔﺮات اﺳﺘﺤﺜﺎثZingiber officinale Roscoe و ﺟﺎﻣﺎ اﺷﻌﺔ ﺑﻮاﺳﻄﺔEMS ﺑﻮار ﻧﻴﻠﻴﺶ* ﻓﺮ ﻧﻤﺒﺎﻟﻜﺮ، ﻣﺎﻧﺴﻨﻜﺮاج ﺑﺎى، ﺳﻨﺪﻳﻴﺐ، دآﺴﺖ ﻗﻨﺸﻢ و آﻮﻻر دوس آﻠﻬﺎﺑﻮر ﺷﻴﻮاﺟﻲ، ﺟﺎﻣﻌﺔ، اﻟﻨﺒﺎت ﻋﻠﻢ ﻗﺴﻢ416004 (MS) اﻟﻬﻨﺪ، اﻟﻤﻠﺨﺺ: ﺟﺎﻧﺐ إﻟﻰ ﺟﺎﻣﺎ أﺷﻌﺔ ﻣﻦ ﻣﺨﺘﻠﻔﺔ ﺟﺮﻋﺎت ﻓﻲ ﻟﻠﻜﻠﻮرﻓﻴﻞ ﻟﻤﺎدة اﻟﻄﻔﺮات ﺗﻮاﺗﺮ ﻧﺴﺒﺔ ﻋﻦ ﺑﻴﺎﻧﺎت اﻟﺒﺤﺚ هﺬا ﻳﺘﻀﻤﻦ EMS اﻟﺰﻧﺠﺒﻴﻞ ﻟﻨﺒﺎت. و ﻣﺨﺘﻠﻔﺔ ﺗﺮددات ﺗﺴﺒﺒﻬﺎ اﻟﺘﻲ اﻟﻄﻔﺮة ﺗﺒﻨﻰ ﺗﻢ وﻗﺪ اﻟﺘﻄﺒﻴﻘﻴﺔ اﻟﻄﺮق ﻣﻦ ﻻﺛﻨﻴﻦ ﺗﺮاآﻴﺰ ﻋﺪة ﺑﻮاﺳﻄﺔ أﻃﻴﺎف اﻟﺪراﺳﺔ هﺬﻩ ﻓﻲ. ﻋﻦ ﻋﺒﺎرة اﻟﻄﻔﺮات أﻃﻴﺎف وآﺎﻧﺖalibino, xantha, and cholrina وﺗﺠﻤﻴﻌﻬﺎ ﻣﻼﺣﻈﺘﻬﺎ ﺗﻢ. ﺑﻴﻨﺖ وﻗﺪ ﻟﻄﻴﻒ اﻟﻄﻔﺮة أن اﻟﻜﻠﻴﺔ اﻟﻨﺘﺎﺋﺞxantha ﻳﻠﻴﻬﺎ ﺗﺮدد ﻷﻋﻠﻲ وﻓﻘﺎ ﺣﺪﺛﺖ اﻟﺰﻧﺠﺒﻴﻞ ﻟﻨﺒﺎتchlorine وalbino اﻟﻨﺘﺎﺋﺞ ﺑﻴﻨﺖ وﻗﺪ اﻟﻤﺎدة ﻓﻌﺎﻟﻴﺔ اﻧﺨﻔﺎض ﺑﻌﺪ وذﻟﻚ اﻟﻤﺘﻄﻔﺮة واﻟﺠﺮﻋﺎت اﻟﻔﻌﺎﻟﻴﺔ ﺑﺒﻴﻦ ﺳﻠﺒﻴﺔ ﻋﻼﻗﺔ ذو اﻟﻤﺘﻄﻔﺮة اﻟﻤﺎدة ﻣﻦ اﻟﺠﺮﻋﺎت اﺧﺘﻼف أن اﻟﺠﺮﻋﺔ زﻳﺎدة ﻣﻊ اﻟﻤﺘﻄﻔﺮة. أن اﻟﻨﻬﺎﺋﻴﺔ اﻟﻨﺘﺎﺋﺞ ﺑﻴﻨﺖ وﻗﺪEMS أﺷﻌﺔ ﻣﻦ أآﺜﺮ اﻟﻜﻠﻮرﻓﻴﻞ ﻟﻤﺎدة اﻟﻄﻔﺮات ﻣﻦ اآﺒﺮ ﻋﺪدا ﻗﺪﻣﺖ ﻓﻲ ﺟﺎﻣﺎ اﻟﻌﻠﻴﻤﺔ اﻟﻮرﻗﺔ هﺬﻩ. ∗ Corresponding Author, Email: nileshsu@gmail.com