_____________________________________________________________________________________________________ *Corresponding author: E-mail: matrix4war2001@yahoo.com; International Journal of Biochemistry Research & Review 23(4): 1-12, 2018; Article no.IJBCRR.44205 ISSN: 2231-086X, NLM ID: 101654445 Phytoremediation of Crude Oil Polluted Agricultural Soil Using Schwenkia americana L. and Spermacoce ocymoides Burm. f. Chukwuemeka C. Chukwuma 1* , Jude C. Ikewuchi 1 , Chimezie Ekeke 2 and Micheal O. Monanu 1 1 Department of Biochemistry, Faculty of Science, University of Port Harcourt, P.M.B. 5239, Choba, Rivers State, Nigeria. 2 Department of Plant Science and Biotechnology, Faculty of Science, University of Port Harcourt, P.M.B. 5239, Choba, Rivers State, Nigeria. Authors’ contributions This work was carried out in collaboration between all authors. All authors read and approved the final manuscript. Article Information DOI: 10.9734/IJBCRR/2018/44205 Editor(s): (1) Dr. Fidanka Trajkova, Assistant Professor, Faculty of Agriculture, Goce Delcev University of Stip, Macedonia. (2) Dr. Mudasir Hafiz Khan, Assistant Professor, Genetics and Plant Breeding, Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar Srinagar (J&K), India. Reviewers: (1) Timur Chis, Ovidius University, Romania. (2) Chris O. Nwoko, Federal University of Technology, Nigeria. (3) Mahmood M. Barbooti, University of Technology, Iraq. (4) Fábio Henrique Portella Corrêa de Oliveira, Universidade Federal Rural de Pernambuco, Brazil. Complete Peer review History: http://www.sciencedomain.org/review-history/26589 Received 26 July 2018 Accepted 08 October 2018 Published 11 October 2018 ABSTRACT Aims: To remove hydrocarbons from crude oil polluted agricultural soil using two selected plant species. Study Design: Mature seeds of Schwenkia americana L. and Spermacoce ocymoides Burm. f. were propagated onto a sterile unpolluted agricultural soil. Seedlings were transplanted into an 8 kg potted homogenised polluted soil for remediation. Place and Duration of Study: Polluted agricultural soil collected from Ogoniland, Rivers State, Nigeria, Ecological Centre of the University of Port Harcourt Nigeria, between May 2017 and February 2018. Methodology: Standard methods were employed for laboratory analyses. Reagents used were of analytical grades with high purity. Original Research Article