1 Supporting Online Material Self-assembly of proteins into designed networks Philippe Ringler and Georg E. Schulz Institut für Organische Chemie und Biochemie, Albert-Ludwigs-Universität Freiburg, Albertstrasse 21, D-79104 Freiburg im Breisgau, Germany Experimental Details Materials The genes of RhuA and PGAL were available from previous experiments ( 1S, 2S). Expression vectors pET3b, pKK223-3 and pUC18 were from Pharmacia (Freiburg) and Stratagene (La Jolla). Point mutations were performed with QuikChange (Stratagene). Oligonucleotides were from MWG (Göttingen). DNA was sequenced by SeqLab (Göttingen). Ready-To-Go T4 DNA ligase was from Amersham (Freiburg). PCR was performed with Pwo polymerase from Sawadi (Erlangen). Restriction enzymes were from MBI-Fermentas (St. Leon-Rot). Bug-Buster-Protein-Extraction reagent was from Novagen (Darmstadt). Centricon concentrators were from Millipore (Schwalbach). Streptavidin and dioleoyl phosphatidylcholine (DOPC) were from Sigma (Steinheim), biotin-HPDP (N-[6- (biotinamido)hexyl]-3'-(2'-pyridyldithio)propionamide) and tris[2-carboxyethyl] phosphine hydrochloride (TCEP) immobilized on agarose was from Pierce (Rockford), 2-(bis- carboxymethyl-amino)-6-[2-(1, 3-di-O-oleyl-glyceroxy)-acetyl-amino] hexanoic acid (Ni- NTA-DOGA) was a gift from P. Schultz (Illkirch). Ni-NTA spin columns were from Qiagen (Hilden). Mutagenesis and Vector Design