Ann Colorectal Res 2021;9(1):32-39. Bioactive Compounds as a Potential Inhibitor of Colorectal Cancer; an insilico Study of Gallic acid and Pyrogallol Debanjan Mitra 1 , Anumita Dey 1 , Ishita Biswas 1 , Pradeep Kumar Das Mohapatra 1 * 1 Department of Microbiology, Raiganj University, Raiganj, WB, India Original Article Introduction: Colorectal cancer (CRC) is one of the most deadly cancers in the world. The objective of this investigation was to evaluate the protective efect of gallic acid and pyrogallol against CRC development. Previous reports suggest that there is an association present between some tannase-producing bacteria and CRC. Tannase is an enzyme that hydrolyzes tannic acid into gallic acid and pyrogallol. The present study aimed to determine the potential therapeutic efect of these compounds in CRC. Methods: The remedial efects of gallic acid and pyrogallol were studied by determining their descriptor properties and applying molecular docking methods. A total of 100 CRC-causing protein structures were docked in this investigation. Results: The Lipinski Rule of Five and other descriptor properties of the mentioned compounds confrmed their non-toxic and therapeutic nature. According to the molecular docking studies, both GA and pyrogallol showed high binding energies with almost all studied proteins, with maximum values of -38.22 kJ/mol and -33.6 kJ/mol being seen for gallic acid and pyrogallol, respectively. Conclusion: This is the frst report on a docking investigation of a large numbers of CRC-related proteins. According to our fndings, we conclude that gallic acid and pyrogallol are protective against CRC as they can block the efects of numerous CRC-causing proteins. Please cite this paper as: Mitra D, Dey A, Biswas I, Das Mohapatra PK. Bioactive Compounds as a Potential Inhibitor of Colorectal Cancer; an insilico Study of Gallic acid and Pyrogallol. Ann Colorectal Res. 2021;9(1):32-39. doi: 10.30476/ACRR.2021.89642.1080. *Corresponding authors: Pradeep Kumar Das Mohapatra Department of Microbiology, Raiganj University, Raiganj, WB, 733134, India Email: pkdmvu@gmail.com Received: 06-01-2021 Revised: 19-04-2021 Accepted: 20-04-2021 Journal compilation © 2020 Annals of Colorectal Research, Shiraz University of Medical Sciences Keywords: Colorectal cancer, descriptor properties, Molecular docking, Binding energy Abstract Introduction C olorectal cancer (CRC) is a very common type of cancer in the world. Worldwide, CRC is ranked second in males and third in females in terms of its frequency. Furthermore, it is ranked fourth in males and third in females for cancer-related death (1). Genetic alteration is reported as one of the causes of CRC progression (2). Many previous studies have revealed that diferent pathways are involved in CRC progression such as the Wnt/β-catenin pathway, TGF-β signaling, PI3K signaling, and many genes that control CRC progression (3). CRC progression is divided into fve stages, namely early adenoma, intermediate adenoma, late adenoma, colorectal carcinoma, and metastasis. The Wnt/β-catenin