www.els-journal.com Page 1 Engineering in Life Sciences Received: 03-Nov-2014; Revised: 10-Jan-2015; Accepted: 02-Feb-2015. This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi: 10.1002/elsc.201400222. This article is protected by copyright. All rights reserved. Short Communication Cell disruption methods for improving lipid extraction efficiency in unicellular microalgae Suchitra Rakesh 1 Dolly W. Dhar 1 Radha Prasanna 2 Anil K. Saxena 2 Supradip Saha 3 Madhulika Shukla 1 Khushbu Sharma 3 1 Centre for Conservation and Utilisation of Blue Green Algae, Indian Agricultural Research Institute (IARI), New Delhi 110012, India 2 Division of Microbiology, Indian Agricultural Research Institute (IARI), New Delhi 110012, India 3 Division of Agricultural Chemicals, Indian Agricultural Research Institute (IARI), New Delhi 110012, India Correspondence: Dr. Dolly Wattal Dhar (dollywattaldhar@yahoo.com), Centre for Conservation and Utilisation of Blue Green Algae, Indian Agricultural Research Institute (IARI), New Delhi 110012, India. Keywords: Biofuels / Cell disruption / Lipid extraction / Microalgae / Microwave treatment Abbreviations: CFPP (cold filter plugging point), ECM (extracellular matrix); FAME (fatty acid methyl esters); GC MS (Gas Chromatography coupled to Mass Spectrometry); LTHT (Pasteurization at Low Temperature Long Time; 62.5°C for 30 min) and HTLT (Pasteurization at High Temperature Short Time: 72°C for 15 sec); UFA (Unsaturated Fatty Acids)