Agricultura – Ştiinţă şi practică no. 3- 4(91-92)/2014 Agriculture - Science and Practice - 144 - ANTIBIO-RESISTANCE STUDY OF STAPHYLOCOCCUS AUREUS STRAINS ISOLATED IN ALGERIAN HOSPITALS Bachir-Pacha M. 1) , A. Bouyoucef 1) , R. R. Triki-Yamani 1) , H. Khaled 1) , F.Teggar 2) , F. Tachet 3) . 1) Institute of Veterinary Sciences-University Blida 2) Département of Biology- University Blida 3) EPH - Blida Abstract. During 06 months (January-June 2013), 109 strains of S. aureus were isolated from 4270 specimens (pus, blood, puncture, CSF) from patients aged from 1 day to 76 years hospitalized in majority (89%). 58.7% of these strains from pus, and 53 strains (48.7%) were MRSA (multi-resistant). The 56 MSSA, less resistant, expressed in the majority of cases, a separated penicillin resistance. Finally, between these 109 strains of S. aureus, none is wild-type. Thus, antibiotic resistance seems still not overcome and the therapeutic arsenal is shrinking. Keywords : Staphylococcus aureus, multi-résistances, MRSA, MSSA INTRODUCTION The starting point of this work is to achieve a phenotypic analysis of S. aureus strains samples isolated from various pathological products for 6 months, in the microbiology laboratory of the CHU Mustapha Bacha (Algiers). For phenotypic analysis of strains, sensitivity against a range of antibiotics (antibiogram) is used routinely in the laboratory. A comparison of phenotypic S. aureus strains resistant (MRSA) and methicillin-sensitive (MSSA) was performed. The objective of this study is the isolation and the identification of S. aureus strains in various pathological products of hospitalized and outpatients; the resistance study of S. aureus strains against certain antibiotics and, the resistance study ofstrains phenotypes and the study of bacteriological characteristics of staphylococcal infections through a prospective study. MATERIAL AND METHODS During 06 months (January to June, 2013), 4270 Samples from outpatients and hospitalized in different services at CHU Mustapha Bacha(Algiers) were analyzed. Material. Samples consist of pus, urine, cerebrospinal fluid (CSF), blood, punctures fluidsand care equipmenst. Internal samples from medical services, emergency and surgical units; external specimens done by private doctors, were made by non- hospitalized patients. Each sample is accompanied by a detailed information sheet. Methods: - Macroscopic examination is done at the reception of specimens and during incubation (10 days). - Cyto-bacteriological examinationallows microscopic identification of live bacteria.The cell count is performed directly by counting under a microscope using a Malassez slide. Finally, staining with bleu of methylene, fast and simple, used to assess the bacterial morphology.