~ 835 ~ Journal of Pharmacognosy and Phytochemistry 2017; 6(3): 835-840 E-ISSN: 2278-4136 P-ISSN: 2349-8234 JPP 2017; 6(3): 835-840 Received: 09-03-2017 Accepted: 10-04-2017 Sarvjeet Department of Genetics and Plant Breeding, (Seed Tech. Section) NDUAT, Kumarganj, Faizabad, Uttar Pradesh, India SC Vimal Department of Genetics and Plant Breeding, (Seed Tech. Section) NDUAT, Kumarganj, Faizabad, Uttar Pradesh, India Jai Prakash Gupta Department of Genetics and Plant Breeding, (Seed Tech. Section) NDUAT, Kumarganj, Faizabad, Uttar Pradesh, India Ashish Singh Research Scholar, Department of Agricultural Meterology, NDUAT, Kumarganj, Faizabad, Uttar Pradesh, India Correspondence Sarvjeet Department of Genetics and Plant Breeding, (Seed Tech. Section) NDUAT, Kumarganj, Faizabad, Uttar Pradesh, India Determination of best combination of bio-fortification and hydro-priming for yield and its quality parameters in chickpea (Cicer arietinum L.) Sarvjeet, SC Vimal, Jai Prakash Gupta and Ashish Singh Abstract The field experiments under present investigation were conducted during Rabi 2014-15 and 2015-16 at Student Instructional Farm and lab experiments were carried out in Seed Testing Laboratory of Seed Technology Section, N. D. University of Agriculture and Technology, Kumarganj, Faizabad (U.P.) with objective of to determine best combination of biofortification and hydropriming for yield contributing traits and seed quality parameters i.e. days to 50% flowering, Days to maturity, Plant height (cm) 30 and 60 DAS, No. of branches per plant 30 and 60 DAS, number of pod/plant, number of seed/pod, Biological Yield (g), harvest index, 100 seed weight (g), seed yield kg/ha, germination (%), seedling length (cm), vigour index, seed yield/plant (g). The experiments were conducted in factorial RBD design with three levels of biofertlizer (Rhizobium 7.5g, 10g, 12.5g, Trichoderma 5g, 7.5g, 10g and PSB 7.5g, 10g, 12.5g) and seed priming (13, 16, 18 hrs) in chickpea variety (Pant G-186), result revealed that inoculation of biofertilizers significantly improved plant height at 30 and 60 days, number of branches per plant on 30 and 60 days, number of pods per plant, number of seeds per pod, biological yield, harvest index, 100- seed weight, seed yield, seed germination, seedling length, vigour index and seed yield per plant. Overall the treatment of Rhizobium dose 12.5g with 16 hrs hydropriming produced higher seed yield (2170.08 Kg) in first year and (2083.17 Kg) in second year, respectively. On the basis of present study it was concluded that nodulation and seed yield of chickpea can be improved by inoculation with Rhizobium and hydropriming 16 hrs which could be economically feasible to increase chickpea production. Keywords: bio-fortification, hydro-priming, Cicer arietinum Introduction Chickpea (Cicer arietinum L.) belongs to genus-Cicer, family-Leguminosae or Fabaceae, and sub family-Papilionaceae. It’s origin is considered in South Eastern Turkey (Ladizinsky, 1975). In India, total production of chickpea was 9.53 million tones from of 9.93 million ha area with average yield of 960 kg/ha in year 2013-14. In area, production and productivity of Uttar Pradesh possessed 577 thousand ha. 475.4 thousand tones, 824 kg ha -1 respectively in year 2013-14 (Anonymous, 2015). Seed priming, several processes including storage, material handling, activation and synthesis of a number of enzymes and nucleic acids, repair and build up, Several biochemical and physiological changes have been observed in seeds during ageing membrane disruption is one of the main reason of seed deterioration the major cause of membrane disruption are increase in free fatty acid level and free radical productivity by lipid peroxydation. Different techniques could be used to enhance crop yield. Priming is also thought to increase free radical scavenging enzymes activity and counteract the effect of lipid peroxidation and reduce leakage of metabolites. The early improvements may increase the rate of unifofmity of seed germination and seedling emergence. (Afzal et al., 2013) [1] The present experiment entitled Determination of best combination of biofortification and hydropriming for yield and its quality parameters in chickpea (Cicer arietinum L.) Materials and Methods The field experiments under present investigation were conducted during Rabi 2014-15 and 2015-16 at Student Instructional Farm and lab experiments were carried out in Seed Testing Laboratory of Seed Technology Section, N. D. University of Agriculture and Technology, Kumarganj, Faizabad (U. P.). Geographically, Narendra Nagar situated between 26.47 0 N latitude, 82.12 0 longitude and at an altitude of 113 meters above the mean sea level. The chickpea crop was sown in the field using randomized block design (Factorial) with eight treatments and three replications. The treatment details are presented in Table 1.