International Journal of Research Studies in Microbiology and Biotechnology (IJRSMB) Volume 6, Issue 1, 2020, PP 1-13 ISSN No. (Online) 2454-9428 DOI: http://dx.doi.org/10.20431/2454-9428.0601001 www.arcjournals.org International Journal of Advanced Research in Microbiology and Biotechnology (IJARSMB) Page | 1 Biosurfactants as Green Biostimulants for Seed Germination and Growth Grazielly Maria Didier de Vasconcelos, Jéssica Mulinari, Vanessa Kristine de Oliveira Schmidt, Renato Dias Matosinhos, José Vladimir de Oliveira, Débora de Oliveira, Cristiano José de Andrade * Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina, Brazil 1. INTRODUCTION The Pre-Sowing Seed Stimulation Is A Technique That Aims To Accelerating The Germination. It Covers The Seed Surfaces With Remarkable Molecules That Protect Them Against Microorganisms And/Or Enhance Their Metabolism, For Example Higher Plant Resistance Against Bacteria, Fungi, Yeasts And Virus. The Seed Stimulation Can Decrease The Initial Dose Of Fertilizers (Krawczyńska Et Al., 2012). In This Sense, Natural-Based Stimulation Methods Have Been Drawn Attention From The World Due To The Their Sustainable Approach. Biosurfactants Is One Of The Most Promissing Molecules As Biostimulants. Biosurfactants Are Amphiphilic Compounds Which Structure Is Composed By Hydrophilic And Lipophilic Moieties. They Are Mostly Derived From Plants And Microorganisms. Nevertheless, The Microbial Production Has Advantages When Compared To Plant-Based Surfactants Due To Their Multifunctional Properties, Rapid Production, And Scale-Up Capacity (Xu Et Al., 2011). In Addition, Their High Biodegradability, High Temperature Stability And Insignificant Effect On Environmental Ph Are Exceptional Advantages For Agricultural Applications. Biosurfactants Can Be Classified Into Five Groups, According To Their Chemical Structure: (I) Lipopeptides And Lipoproteins, (II) Glycolipids, (III) Fatty Acids, Neutral Lipids And Phospholipids, (IV) Polymeric Surfactant And (V) Particulate (Andrade Et Al., 2016a). Lipopeptides And Glycolipids Are The Most Scientifically Well- Known Biosurfactants. As Already Mentioned, Lipopeptides Have Been Exhaustively Investigated, In Which Bacillus Subtilis Lipopeptides Such As Surfactin, Iturin And Fengycin Families Have Risen To Prominence. In 2003, The Use Of Daptomycin, A Semi-Synthetic Cyclic Lipopeptide, As Antibiotic Was Approved In The USA. The Chemical Amphiphilic Structure Of Surfactin, Iturin And Fengycin Families Is Basically Composed Of A Cyclic Peptide Linked To Fatty Acid Chain, Β-OH (Lactone), Β-NH 2 (Lactam) And Β-OH (Lactone), Respectively. The Peptide Moiety Of The Surfactin And Iturin Families Contains A Heptapeptide Whereas The Fengycin Family, A Decapeptide. It Is Worth Noting That Subtle Chemical Structural Differences Are The Key For Biological Activities Of Lipopeptides *Corresponding Author: Cristiano José de Andrade, Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina, Brazil. . Abstract: Microorganisms, particularly fungi, are well-known to significantly affect crop production. In 2011, the United States produced over 35 million tons of grains. Grain rust diseases were estimated to lead a US$5 billion loss. Thus, to enhance the crop production, pesticides are extensively used in agriculture, most synthetic ones. Consequently, humans are often exposed to pesticides mainly by residues in food and water. In this sense, natural-based pesticides have been drawn attention from the world due to their sustainable approach. Biosurfactants is one of the most promising molecules as green pesticides. In addition, biosurfactants have biostimulants properties such as metabolism enhancers. This review paper highlights the key features in biosurfactants, in particular lipopeptides and glycolipids as green biostimulants for seed germination and growth. In conclusion, on the one hand, lipopeptides, rhamnolipids and sophorolipids have already been studied as biostimulants; on the other hand mannosylerythritol lipids have been poorly investigated - mostly due to their antimicrobial properties. Keywords: Lipopeptides, Glycolipids, Biostimulants.