Int.J.Curr.Microbiol.App.Sci (2018) 7(10): 3379-3386 3379 Original Research Article https://doi.org/10.20546/ijcmas.2018.710.391 Antibacterial Activity of Electrochemically Activated Water Solutions on Pseudomonas aeruginosa after Four Weeks Storage Teodora P. Popova * and Toshka E. Petrova University of Forestry, Faculty of Veterinary Medicine, 10 Kl. Ohridski Blvd., Sofia 1756, Bulgaria *Corresponding author ABSTRACT Introduction The increasing prevalence of strains of pathogenic bacteria showing multiple resistance to antibiotics and also the rapid development of resistance to commonly used disinfectants are serious problems on a global scale. On the other hand, the pollution of the environment with chemical agents used to combat microorganisms in all spheres of human activity is growing. This leads to disturbance of the ecological balance and biodiversity in the nature. In recent years, the electrochemically activated aqueous solutions (EAASs) have been reported to be broad- spectrum and environmentally safe biocidal products. The scope of their action includes not only bacteria but also spores, viruses and fungi. Gurgulova et al., (2010, 2011), Popova et al., (2016a, 2016c) and others found significant antibacteral activity of such solutions against Gram-positive and Gram- negative bacteria. Atanasov et al., (2014), Karadzhov et al., (2014) and other authors reported for antiviral activity of EAASs, and Tasheva et al., (2010) - for their antimycotic action. Antimicrobial effect of such solutions was found even in environments with high protein content such as biosludge (Dimitrova et al., 2013) and water from lagoons for storing bovine manure (Peev, 2017). The Gram-negative bacteria exhibit higher International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 7 Number 10 (2018) Journal homepage: http://www.ijcmas.com Studies have been carried out to determine the effect of activated aqueous solutions (anolytes and catholytes) on multi-resistant strain of Pseudomonas aeruginosa in vitro after storage at room temperature. The solutions were obtained by electrochemical activation of water with 0.8% NaCl, and with a combination of 0.4% NaCl and 0.4% Na 2 CO 3 . The disinfectant Virkon S was used as a control. Cultures were made on Cetrimide agar after different intervals of action of the solutions on P. aeruginosa suspensions (10 6 cells/ml). Anolytes and catholytes tested exhibited high antimicrobial activity. Upon potentiation of the anolytes by addition of 96% ethanol with a final concentration of 1%, bactericidal activity was observed within 2 minutes. The use of catholyte of NaCl to dissolve Virkon S resulted in an increase in its effect compared to its use as an aqueous solution. After storage for 29 days, the solutions tested preserved their antibacterial properties to the maximum extent. Keywords Antibacterial activity, Anolyte, Catholyte, Pseudomonas aeruginosa Accepted: 24 September 2018 Available Online: 10 October 2018 Article Info