How to Cite: Aggarwal, M., Sharma, M., Karthikeyan, R., Kumar, M., Chawla, G., & Tyagi, V. (2022). A comparative study of honey, jaggery an ethanol as cytological fixatives. International Journal of Health Sciences, 6(S3), 3902–3914. https://doi.org/10.53730/ijhs.v6nS3.6651 International Journal of Health Sciences ISSN 2550-6978 E-ISSN 2550-696X © 2022. Manuscript submitted: 27 Dec 2021, Manuscript revised: 09 March 2022, Accepted for publication: 18 April 2022 3902 A comparative study of honey, jaggery an ethanol as cytological fixatives Dr. Manish Aggarwal Third year post graduate student, Department of Oral pathology and microbiology, Surendera Dental College and Research Institute, Sri Ganganagar, Rajasthan, India Dr. Manish Sharma Prof & Head, Department of Oral Pathology and Microbiology, Surendera Dental College and Research Institute, Sri Ganganagar, Rajasthan, India Dr. R. Karthikeyan Prof & Head, Saveetha Dental College, Chennai, India Dr. Manish Kumar Reader, Department of Oral pathology and microbiology, Surendera Dental College and Research Institute, Sri Ganganagar, Rajasthan, India Corresponding author email: dr.manishk84@gmail.com Dr. Gurveen Chawla Senior lecturer, Department of Oral pathology and microbiology, Surendera Dental College and Research Institute, Sri Ganganagar, Rajasthan, India. Dr. Vineet Tyagi MDS Oral Pathology, General Practitioner, New Delhi, India Abstract---The processing of cytological samples. It is the gold standard cytological fixative used in many laboratories. However, it is an expensive, volatile and flammable liquid with an irritant smell. It has also been shown to be carcinogenic in some animal models. Hence, a need to identify a safer substitute of alcohol cytological fixative is necessary. The aim of study was to analyse the efficacy of cytological smears fixed in ethanol & 20% unprocessed honey and 30% jaggery solution and to compare the efficacy between the three fixatives on staining. Three buccal smears from 50 healthy volunteers each, were collected from either cheek or tongue using wooden spatula. One of the smear was fixed in ethanol (95%) and Rapid Papanicolaou staining was done. And the other two smear was fixed in honey (20%) and Jaggery (30%) and Rapid Papanicolaou staining was done. The cytoplasmic and nuclear details were evaluated using