Open Peer Review Discuss this article (0) Comments RESEARCH ARTICLE Antimicrobial of nyirih ( against Xylocarpus granatum) pathogens of tiger shrimp post-larvae [version 1; referees: awaiting peer review] Gina Saptiani , A. Syafei Sidik , Fikri Ardhani 2 Aquatic Microbiology Laboratory of Fisheries and Marine Sciences Faculty, Mulawarman University, Samarinda, Kalimantan Timur, 75119, Indonesia Animal Science, Agriculture Faculty, Mulawarman University, Samarinda, Kalimantan Timur, 75119, Indonesia Abstract has been used as a medicinal plant by Background: Xylocarpus granatum coastal communities, which may indicate that this plant is a potential source of pharmaceuticals. leaf extract was tested as an antimicrobial Methods: Xylocarpus granatum agent for pathogens infecting tiger shrimp post-larvae. Of the treatments applied to the post-larvae, 25 were crudely extracted with ethanol, distilled water, and seawater solvent given by immersion. and Vibrio harveyi sp. were microbial species used for the test. Saprolegnia extract had the potential to inhibit and Results: X. granatum V. harveyi sp., reducing infection and improving the survival of shrimp. Saprolegnia Shrimp soaked with extract had a total count ranging from X. granatum Vibrio 14.67x10 to 22.67x10 CFU/ml. The survival rate of shrimp was recorded as 53.33% to 78.67% and 54.67% to 76.00% due to , and V. harveyi Saprolegnia sp infection, respectively. The relative percentage of the survival of shrimp protected from and sp infection in treatments compared V. harveyi Saprolegnia to negative controls ranged from 40.61% to 72.89% and 35.84% to 66.12%, respectively. Leaf extracts of , which might have better Conclusions: X. granatum antimicrobial activities to prevent tiger shrimp from pathogenetic infection, were consecutively extracted ethanol at 800-1,000 ppm, distilled water at 800-1,000 ppm, and seawater at 1,000 ppm. Keywords antimicrobial, pathogen, tiger shrimp, Xylocarpus granatum 1 1 2 1 2 Referee Status: AWAITING PEER REVIEW 16 Jan 2019, :63 ( First published: 8 ) https://doi.org/10.12688/f1000research.16653.1 16 Jan 2019, :63 ( Latest published: 8 ) https://doi.org/10.12688/f1000research.16653.1 v1 3 3 Page 1 of 9 F1000Research 2019, 8:63 Last updated: 16 JAN 2019