Kragujevac J. Sci. 36 (2014) 145-154. UDC 581.192:582.923:615.33 ANTIMICROBIAL ACTIVITY AND SOME PHYTOCHEMICAL ANALYSIS OF TWO EXTRACTS Vinca minor L. Sandra M. Grujić 1* , Ivana D. Radojević 1 , Sava M. Vasić 1 , Ljiljana R. Čomić 1 and Marina D. Topuzović 2 1 Laboratory of Microbiology, 2 Laboratory of Botany, Department of Biology and Ecology, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac, Republic of Serbia *Corresponding author. E-mail: sandragrujic89vp@gmail.com (Received April 3, 2014) ABSTRACT. This study investigated the antimicrobial activity as well as some phytochemical analysis of ethanol and diethyl ether extracts from plant species Vinca minor L. In vitro antimicrobial activity of extracts was studied on 20 strains of microorganisms (16 bacteria and four yeasts). Testing was performed by microdilution method and minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) were determined. The strongest antimicrobial activity was detected on G+ bacteria of the genus Bacillus. Tested G - bacteria and yeasts were not sensitive to the action of the extracts or the sensitivity was insignificant. Phytochemical analysis involved determining the amount of total phenolics, flavonoids and tannins, as well as the determination of antioxidant activity monitoring capability to neutralize free radicals (DPPH) and the reductive potential. Phytochemical examination indicates that the total phenolic compounds were more in the ethanolic extract and the content of flavonoids and tannins marginally higher in the diethyl ether extract. The antioxidant activity (DPPH) of the ethanolic extract of V. minor was significantly stronger as compared to the diethyl ether extract, and the reduction potential was approximately the same. Keywords: Vinca minor, antimicrobial activity, antioxidant activity, phenols, flavonoids, tannins. INTRODUCTION The genus Vinca (Apocynaceae) comprises about seven species in the world. In Serbia, it is represented by Vinca herbacea, Vinca minor and Vinca major. V. minor or Lesser Periwinkle occurs throughout central Serbia (JOSIFOVIĆ eds., 1973). The leaves are oblong-ovate to elliptical, approximately 2-5 cm long and 1-2.5 cm wide, with petioles 1-3 mm long. The flower is perfect and without odor. It is showy, purple to blue and occasionally white, borne singly in an axillary position on a 1-1.5 cm pedice. The fruit is a non-fleshy follicle, approximately 2–2.5 cm long. It releases three to five seeds (DARCY et al., 2002). V. minor flowers regularly from April to May and sporadically from May to September (FUNDERBUCK et al., 1976). It is adapted to the mild climate. It grows best in partial shade and moist, well-drained soil. It is often found in woods and bluffs, and along cemeteries, roads, and other disturbed areas (KURT and CAROL, 2000). In folk medicine, it is used internally for