Pak. J. Bot., 40(5): 1913-1921, 2008. ANALYSIS OF FATTY ACID, ELEMENTAL AND TOTAL PROTEIN OF CALOTROPIS PROCERA MEDICINAL PLANT FROM SINDH, PAKISTAN SAMINA KABIR KHANZADA, W. SHAIKH, T.G. KAZI, S. SOFIA, AMINA KABIR, K. USMANGHANI AND AFTAB A. KANDHRO Institute of Botany, University of Sindh, Jamshoro-76080, Pakistan National Center of Excellence for Analytical Chemistry, University of Sindh, Jamshoro, Pakistan. Department of Basic Clinical Sciences, Faculty of Eastern Medicine, Hamdard University, Karachi, Government Degree Girls College Moro, PCSIR Labs. Complex, Lahore-54600, Pakistan Abstract Calotropis procera (Asclepiadaceae) is a well known medicinal plant with leaves, roots and bark being exported as popular medicine to fight many human and animal diseases. It is locally known as AKK with English name as Milk Weed, grows abundantly in Sindh province of Pakistan. The isolated fatty acid composition in the extract of C.procera has 7 saturated fatty acid and 11 unsaturated fatty acid. The essential elements Al, As, Cu, Ca, Cr, Cd, Fe, K, Mn, Na, Pb, and Zn have been analyzed from the medicinal plant in variable range. The total protein in C. procera was 27-32% Introduction Calotropis procera (Ait). R.Br. of the family Ascelpiadaceace is locally known as Akk.The latex of the plant is used as an antidysenteric, antirheumatic, a diaphoretic, an expectorant and for the treatment of bronchial asthma and skin conditions (Ageel, 1987; Al- Yahya et al., 1990). In African and Asian countries, the latex of C. procera is utilized as an arrow poison molluscide, a fungicide, an anti-syphilitic, an anti-inflammatory, a purgative, for the treatment of lepers and bronchial asthma and for milk coagulation in chease making (Watt & Breyer-Brandwijik, 1962; EL`Badwi, 1997; Larhsinil, 1997). C. procera is known to contain cardio active glycoside calotropine which has shown an antitumer effect in vitriol on human epidermoid carcinoma cells of the rhinopharynx, it also acts as expectorant and diuretic. C. procera is used as expectorant and anthelmintic. Milky juice is reported for the treatment of dropsy and rheumatism to remove Taenia, the treatment of toothache. (Kalita, 2001). However dried leaves are smoked in pipe as cure for cough (Aftab et al., 1990; Csurhes et al., (1998). C. procera is considered for large scale cultivation as an alternative source for producing energy (Hifzul Kabir, 2003); Calotropis procera is applied for the treatment of expectorant, analgesic and anti inflammation (Dhiman, 2003) C. procera is used for digestion. Oil of leaves is useful in skin eruptions. Latex from C. procera, is widely used in folk medicine as a rich source of biologically active compounds capable of promoting diverse benefits such as control of dermal fungal infections, antimicrobial activities and pain relief among other useful properties. The identification and cultivation of plants rich in hydrocarbons as renewable sources of chemicals for use as fuel and chemical feedstock has generated considerable interest (Nielsen et al., 1977; Buchanan et al., 1978a, b; Calvin, 1978; Saxon, 1980; Wang & Hu.man, 1981; Adams & Machesney, 1982; Campbell, 1983; Jenkins and Ebeling, 1985; Abbott et al., 1990; Seiler et al., 1991). The