Research Article
Investigating Potential Inhibitory Effect of Uncaria tomentosa
(Cat’s Claw) against the Main Protease 3CL
pro
of SARS-CoV-2 by
Molecular Modeling
Andres F. Yepes-P´ erez ,
1
Oscar Herrera-Calderon ,
2
Jos´ e-Emilio S´ anchez-Aparicio ,
3
Laura Tiessler-Sala ,
3
Jean-Didier Mar´ echal ,
3
and Wilson Cardona-G
1
1
Chemistry of Colombian Plants, Institute of Chemistry, Faculty of Exact and Natural Sciences, University of Antioquia-UdeA,
Calle 70 No. 52-21, A.A 1226, Medellin, Colombia
2
Academic Department of Pharmacology, Bromatology and Toxicology, Faculty of Pharmacy and Biochemistry,
Universidad Nacional Mayor de San Marcos, Jr Puno 1002, Lima 15001, Peru
3
Insilichem, Departament de Qu´ ımica, Universitat Aut` onoma de Barcelona, Edifici C.n., 08193 Cerdanyola del Vall´ es,
Barcelona, Spain
CorrespondenceshouldbeaddressedtoAndresF.Yepes-P´ erez;andresf.yepes@udea.edu.co,OscarHerrera-Calderon;
oherreracalderon@gmail.com,andJean-DidierMar´ echal;jeandidier.marechal@uab.cat
Received 7 May 2020; Accepted 25 July 2020; Published 1 October 2020
GuestEditor:ZhengFeeiMa
Copyright©2020AndresF.Yepes-P´ erezetal.isisanopenaccessarticledistributedundertheCreativeCommonsAttribution
License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is
properly cited.
COVID-19isadiseasecausedbysevereacuterespiratorysyndromecoronavirus2.Presently,thereisnoeffectivetreatmentfor
COVID-19. As part of the worldwide efforts to find efficient therapies and preventions, it has been reported the crystalline
structureoftheSARS-CoV-2mainproteaseM
pro
(alsocalled3CL
pro
)boundtoasyntheticinhibitor,whichrepresentsamajor
druggabletarget.edruggabilityofM
pro
couldbeusedfordiscoveringdrugstotreatCOVID-19.Amultilevelcomputational
studywascarriedouttoevaluatethepotentialantiviralpropertiesofthecomponentsofthemedicinalherb Uncaria tomentosa
(Cat’sclaw),focusingontheinhibitionofM
pro
.e in silico approachstartswithprotein-liganddockingof26Cat’sclawkey
components,followedbyligandpathwaycalculations,moleculardynamicssimulations,andMM-GBSAcalculationofthefree
energyofbindingforthebestdockedcandidates.estructuralbioinformaticsapproachesledtoidentificationofthreebioactive
compounds of Uncaria tomentosa (speciophylline, cadambine, and proanthocyanidin B2) with potential therapeutic effects by
strong interaction with 3CL
pro
. Additionally, in silico drug-likeness indices for these components were calculated and showed
good predicted therapeutic profiles of these phytochemicals. Our findings suggest the potential effectiveness of Cat’s claw as
complementary and/or alternative medicine for COVID-19 treatment.
1. Introduction
Severe acute respiratory syndrome coronavirus 2 (SARS-
CoV-2) is a part of coronavirus (CoV) family and was
initiallyidentifiedinWuhan,China,attheendofDecember
2019.COVID-19ishighlycontagiousandismostfrequently
transmitted from human to human, spreading the virus
easilytoothercountriesinaveryshorttime[1].According
tothelastreportoftheWorldHealthOrganization(WHO),
the severe acute respiratory syndrome (coronavirus disease
(COVID-19)) caused by SARS-CoV-2 is considered a
pandemic,affectingAsiaandEuropewiththehighestdeath
ratefollowedbyAmericaandotherregions,causingserious
public health problems and considerable economic losses
worldwide [2]. Respiratory viral infections are the frequent
causes of morbidity and millions of hospital admissions in
developing countries every year [3]. For this reason, the
pharmacotherapy based on natural products may be a
proper alternative for treating viral diseases. On the other
hand, traditional medicine is practiced by native South
Hindawi
Evidence-Based Complementary and Alternative Medicine
Volume 2020, Article ID 4932572, 14 pages
https://doi.org/10.1155/2020/4932572