Abstracts / Journal of Clinical Virology 70 (2015) S1–S126 S49 Abstract No: 1600 Presentation at ESCV 2015: Poster 1 A measles death in Wales – An inevitable yet preventable consequence of a large measles outbreak C. Moore 1,* , J. Hoffmann 1 , M. Brotto 2 , R. Jones 1 1 Public Health Wales, United Kingdom 2 ABMU Health Board, United Kingdom Background: A now well documented measles outbreak occurred in Wales during 2012–2013. As the measles outbreak approached 1000 cases, the chances of a fatality associated with the outbreak increased as estimates put case fatality rates in regions with good access to healthcare at 0.1%. On 18th April 2013, a body of a 25 year old man was discovered at his home in Swansea. In the days leading up to the discovery, the case had been in contact with primary care due to developing a fever and rash. Methods: Initial molecular testing of a throat swab and EDTA blood was undertaken prior to autopsy to confirm measles virus infection. Cause of death was confirmed by targeted swabbing of the respiratory tract and internal organs during post mortem. This was then followed up by histology. Results: measles virus RNA was confirmed in samples collected both prior to autopsy and from swabs collected during the autopsy. Despite several days passing from death to autopsy, high levels of measles RNA was demonstrated throughout the respiratory tract. Histology later confirmed giant cell pneumonia. Conclusions: This case was covered extensively by both con- ventional and social media where doubts were cast about whether measles was the cause of death due to other lifestyle factors. We take this opportunity to highlight the dangers of measles virus infection and to demonstrate how measles caused the death of this young man. http://dx.doi.org/10.1016/j.jcv.2015.07.116 Abstract No: 1601 Presentation at ESCV 2015: Poster 1 Molecular epidemiology of respiratory syncytial virus between 2010–2015, in Portugal R. Guiomar * , P. Cristóvão, P. Conde, P. Pechirra Portuguese Influenza Reference Laboratory. National Institute of Health Dr. Ricardo Jorge, Porto, Portugal Background: Respiratory syncytial virus is one of the major causes of respiratory infection and complications in younger chil- dren and elderly. This study has, for the first time, investigated the genetic diversity of RSV A and RSV B detected since 2010, in influenza like illness (ILI) cases reported in the scope of the Por- tuguese Influenza Surveillance Programme (NISP). Methods: During 2010–2015, nasopharyngeal swabs (NPS) sent to the National Influenza Reference Laboratory from sentinel and non-sentinel network were tested for RSV A and RSV B by real time multiplex RT-PCR. Nucleotide sequence of a fragment of the hypervariable C-terminal region of the G protein gene and the phy- logenetic analysis was performed for a half of detected RSV. Results: Over the study period were detected 114 (5.2%) RSV in 2187 tested NPS. Of these 67 (59%) were from subtype A and 47 (41%) from subtype B. Circulation of RSV preceded or was coinci- dent with the influenza epidemic period. RSV A was predominant in each winter with exception for 2014/2015 winter when RSV B was predominantly detected. Of the RSV positive samples, 58 (51%) were successfully sequenced and genetically characterized: 26 (45%) RSV A and 32 (55%) RSV B. RSV A clustered in two geno- types. A majority (n = 22; 85%) belonged to ON1 genotype and 4 (15%) viruses belonged to NA1 genotype. Only ON1 genotype was detected after 2012/2013 season. RSV B clustered in two genotypes: a majority (n = 22; 67%) belonged to BA9 genotype and 11 (33%) clustered in BA10 genotype. BA9 genotype was detected over all the study period, although BA10 was only detected in 2012/2013, and 2014/2015 seasons. Conclusion: Our study highlights the importance of RSV in ILI cases, showing a seasonal circulation each winter season during influenza epidemic. RSV accounted for 5.2% of the cases reported in the scope of influenza surveillance, assuming a huge importance in young children and older ones. Molecular data for RSVA revealed co circulation of NA1 and ON1 till 2012, and after this period ON1 was exclusively detected suggesting a strain replacement by this antigenically advantageous genotype. Globally ON1 is also predom- inantly detected. For RSVB subtype was observed a co circulation of the BA9 and BA10 genotypes. BA derived genotypes, first identified in 1999 in Buenos Aires are predominant in many countries since then. http://dx.doi.org/10.1016/j.jcv.2015.07.117 Abstract No: 1606 Presentation at ESCV 2015: Poster 1 Early detection of adenovirus reactivation in a stem cell transplant recipient: The value of using stool samples M. Echavarría 1,* , D. Torres 2 , D.N. Marcone 1 , M. Labadie-Bracho 1 , V. Romano 3 , F. Herrera 2 1 Virology Unit, CEMIC, Buenos Aires, Argentina 2 Infectology Section, CEMIC, Buenos Aires, Argentina 3 Virology Laboratory, CEMIC, Buenos Aires, Argentina Background: Adenoviruses (AdV) can cause disseminated dis- ease that can lead into a fatal outcome in stem cell transplant recipients. Patients at risk are usually screened for the presence of AdV in blood. Recent studies have proposed the use of stool samples for an earlier AdV detection. Patient and methods: A 55 year-old man with acute myeloblas- tic leukemia diagnosed in October 2013 received an allogeneic hematopoietic stem cell transplant on 28th May 2014. Condi- tioning regimen included fludabarin/busulfan. The engraftment was achieved on day 13, without complications and cyclosporine was started to prevent graft-versus-host disease (GVHD). Antimi- crobial prophylaxis included trimethoprim-sulfamethoxazole and valacyclovir. Bone marrow biopsy performed at day 100 showed disease remission. Cyclosporine was discontinued. On day 137, the patient was readmitted for severe diarrhea and weakness. Digestive endoscopy showed multiple ulcers in duodenum and colon. GVHD was suspected and methylprednisolone pulses were started. Prophylaxis with voriconazole, TMS and acyclovir was indi- cated. On day 155 he presented gastrointestinal bleeding and acute renal failure requiring hemodialysis. On day 160 he presented febrile neutropenia with abdominal source. Bacterial blood cul- tures were negative. Meropenem, daptomycin and colistin were started. The patient died on day 166 with septic shock and mul- tiple organ failure. Duodenum and colon biopsies were obtained as well as sequential plasma and stool samples for viral detection. Viral nucleic acids were extracted using DNA extraction columns (QIAGEN ® ). AdV detection was performed with a conventional PCR and a commercial quantitative real-time PCR (AdV R-gene kit, bioMérieux ® ). AdV typing was performed by a real-time PCR with specific probes for AdV species A/C, B, E and F. Serotyping is pending.