J Anim Breed Genet. 2019;00:1–19. wileyonlinelibrary.com/journal/jbg
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1 © 2019 Blackwell Verlag GmbH
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INTRODUCTION
The selection for growth traits using phenotypes and pedigree
data with best linear unbiased prediction (BLUP) (Henderson,
1975) has been effective over the years. However, this can be
improved if DNA polymorphisms affecting growth traits in
beef cattle are determined (Snelling et al., 2010). Genome-
Wide Association Studies (GWAS) enable the identification
of SNPs associated with the quantitative trait loci (QTL) of
interest and/or genes related to the phenotypic expression of
the trait (Utsunomiya et al., 2013). The assumption in GWAS
is that association arise because at least one single nucleotide
Received: 6 August 2019
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Revised: 3 November 2019
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Accepted: 5 November 2019
DOI: 10.1111/jbg.12458
ORIGINAL ARTICLE
Tag-SNP selection using Bayesian genomewide association study
for growth traits in Hereford and Braford cattle
Gabriel Soares Campos
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Bruna Pena Sollero
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Fernando Antonio Reimann
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Vinicius Silva Junqueira
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Leandro Lunardini Cardoso
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Marcos Jun Iti Yokoo
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Arione Augusti Boligon
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José Braccini
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Fernando Flores Cardoso
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Departamento de Zootecnia, Universidade
Federal de Pelotas, Pelotas, Brazil
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Embrapa Pecuária Sul, Bagé, Brazil
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Departamento de Zootecnia, Universidade
Federal de Viçosa, Viçosa, Brazil
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Departamento de Zootecnia, Universidade
Federal do Rio Grande do Sul, Porto
Alegre, Brazil
Correspondence
Gabriel Soares Campos, Universidade
Federal de Pelotas, Departamento de
Zootecnia, Pelotas, RS, 96010-900, Brazil.
Email: gabrielsoarescampos@hotmail.com
Funding information
Conselho Nacional de Desenvolvimento
Científico e Tecnológico, Grant/Award
Number 305102/2018-4; Empresa
Brasileira de Pesquisa Agropecuária, Grant/
Award Number 12.13.14.014.00.
Abstract
The aim of this study was to perform a Bayesian genomewide association study
(GWAS) to identify genomic regions associated with growth traits in Hereford and
Braford cattle, and to select Tag-SNPs to represent these regions in low-density pan-
els useful for genomic predictions. In addition, we propose candidate genes through
functional enrichment analysis associated with growth traits using Medical Subject
Headings (MeSH). Phenotypic data from 126,290 animals and genotypes for 131 sires
and 3,545 animals were used. The Tag-SNPs were selected with BayesB (π = 0.995)
method to compose low-density panels. The number of Tag-single nucleotide poly-
morphism (SNP) ranged between 79 and 103 SNP for the growth traits at weaning
and between 78 and 100 SNP for the yearling growth traits. The average proportion
of variance explained by Tag-SNP with BayesA was 0.29, 0.23, 0.32 and 0.19 for
birthweight (BW), weaning weight (WW205), yearling weight (YW550) and post-
weaning gain (PWG345), respectively. For Tag-SNP with BayesA method accuracy
values ranged from 0.13 to 0.30 for k-means and from 0.30 to 0.65 for random clus-
tering of animals to compose reference and validation groups. Although genomic
prediction accuracies were higher with the full marker panel, predictions with low-
density panels retained on average 76% of the accuracy obtained with BayesB with
full markers for growth traits. The MeSH analysis was able to translate genomic
information providing biological meanings of more specific gene products related to
the growth traits. The proposed Tag-SNP panels may be useful for future fine map-
ping studies and for lower-cost commercial genomic prediction applications.
KEYWORDS
beef cattle, genomic prediction, GWAS, low-density panel