Open Access, Volume - 1
Alpha2- Adrenergic blockers restore noise-induced biochemical and
cognitve disorders
Manukyan A. L*; Hunanyan L.S; Melkonyan M.M
Department of Medical Chemistry Yerevan State Medical University afer M. Heratsi, Armenia.
1
Research Artcle
*Corresponding Author: Manukyan Ashkhen, Assistant Professor, De-
partment of Medical Chemistry, Yerevan State Medical University afer
M. Heratsi, Koryun 2, 0025, Yerevan, Armenia, Tel: +374 77 011740.
Email: manukyanashkhen@mail.ru, ashkhmanuk@mail.ru.
Received Date : Oct 04, 2021
Accepted Date : Nov 03, 2021
Published Date : Nov 10, 2021
Archived : www.jcmimagescasereports.org
Copyright : © Manukyan Ashkhen 2021.
Abstract
Noise-induced stress causes central nervous system dysfuncton and neurotransmission impairment in the brain and changes
hormone levels which potentally result in psychological and behavioral problems. Considering the signifcance of the poten-
tal negatve outcome of the chronic stress, the aim of our investgaton was to study the level of inositol triphosphate (IP3)
in the mitochondrial fracton of the brain (MFB), behavioral characteristcs and the efects of the α2-adrenoblockers beditn
and mesedin to reveal their antoxidatve efect during noise exposure. The open feld tests were used in order to evaluate the
behavioral states of the rats. Investgatons were carried out on albino rats divided into 4 groups. The 1st group of rats served
as a control. The 2nd, 3rd and 4th groups were exposed to 91 dBA of noise; the duraton of exposure was 8 hours per day for
60 days. The 3rd group was injected with beditn and the 4th group with mesedin, both intraperitoneally and repeatedly. Ac-
cording to our results, the chronic exposure to high-level noise leads to the decreased level of IP3 resultng development of
oxidatve stress and metabolism imbalance. The open feld test revealed that chronic noise exposure caused noise duraton-
dependent delay in the locomotor and behavioral actvity. Intensity of these changes were dependent on the duraton of
noise exposure. The administraton of α2-adrenoblockers to the noise-exposed animals had a regulatory efect on the studied
parameters under the conditons of chronic acoustc stress.
Keywords: Noise, α2-adrenoblockers, mitochondrial fracton of the brain, inositol triphosphate, anxiety.
Volume 1 | Issue 1 | 2021
Background
Noise impact has been demonstrated to induce a variety of
physiological efects in biological systems [1]. Noise-induced
stress promotes enhancement of the free oxygen radical’s in-
tensity because of high respiratory oxygen intake and meta-
bolic turnover. Cells contnuously produce free radicals and
reactve oxygen species (ROS) as a result of their metabolic
processes [2,3]. Lipid peroxidaton is a free-radical-mediated
chain of reactons that, once initated, results in oxidatve
damage of polyun¬saturated lipids. So, the most targets for
this kind of acton are becoming the components of biolo¬gical
membranes. These reactons can be initated or enhanced by
a number of toxic products, including endoperoxides and al-
dehydes [4,5]. It was asserted that noise expositon in early
stages produces long-term cognitve impairment and damage
to the hippocampal neurons [6]. At present it is highly actual
to fnd the ways of protecton from the stress impact, con-
sidering its deterioratve acton on the organism’s diferent
organs and systems. In a search for new alternatves for α2-
adrenergic blockers, 1,4-benzodioxane derivatves mesedin
(2-(2-methylamino-4-thiozolyl) -1,4-benzodioxan hydrochlo-
ride) and beditn (2-amino-4-thiozolyl -1,4-benzodioxan), have
been chosen for the further studies because of being selectve
and stable blockers of the peripheral α2-adrenoreceptors and
less toxic.
Taking into account the important regulatory functon of phos-
phoinositds (PI) in the signaling system and ensuring mem-
brane functons, we studied the content of IP3 as a minor
fracton of membrane phospholipids (PL) in the experimental
animals’ brain mitochondria and behavioral actvity ie. anxi-
ety.
Material and Methods
Animals
Investgatons have been carried out on albino male rats aged
5-6 weeks, kept in ordinary vivarium conditons, maintained
on a 12 h light/dark cycle with food and water and libitum
in accordance with the European Communites Council Di-
rectve (86/609/EEC) on care and use of animals for experi-
mental procedures. The following specifc in vivo conditons
were kept for each experiment: 200C temperature and 52%
humidity. The animals were accommodated to the laboratory
conditons for 7 days prior to the experiment. The rats were
grouped into 4 groups (6 per group). The 1st group was a con-
trol; the 2nd group (noise) was just exposed to noise, the 3rd