densitometric (DEXA) method. Using an INSTRON 3367 apparatus (Instron, USA) and three-point bending test, mechanical parameters such as maximum elastic strength (Wy) and ultimate strength (Wf) of mandible were estimated. Pearson's correlation coefficient (r) was determined between all the investigated variables and P b 0.05 was considered as statistically significant. Obtained results showed significant positive correlation between body weight and mandible weight, lenght, Bvol, Cd, BMD, BMC, Wy and Wf (P b 0.05). All the investigated parameters of the mandible were found to be positively correlated. However, statistically insignificant correlations of MvBMD and body weight, mandible weight, mandible length and Bvol were stated. Furthermore, Bvol and Cd were not found to be significantly correlated (P N 0.05). In conclusion, this study showed numerous positive correlations between final body weight, densito- metric, morphometric and mechanical properties of mandible. This bone of pigs may be used as an attractive model for further investigations on metabolic response of skeleton to physiological, nutritional, toxicological and pharmacological factors influencing bone tissue metabolism. This article is part of a Special Issue entitled ECTS 2012. Disclosure of interest: None declared. doi:10.1016/j.bone.2012.02.342 PP154 Detection of osteoadherin during intramembranous and endochondral ossification by colloidal gold immunocytochemistry and Western blotting D.S. Janones a, ⁎, T.A.G. Donato a , V. Bradaschia-Corrêa a , F. Bleicher b , V.E. Arana-Chavez a a Biomaterials and Oral Biology, Universaty of São Paulo, São Paulo, Brazil b Laboratoire Odontoblast et régénération dentinaire, University of Lyon, Lyon, France Abstract: Osteoadherin (OSAD) is a keratan sulfate proteoglycan firstly isolated from bovine bone. Although the distribution of OSAD in mineralized tissues had been described suggesting a role in biomineralization, its specific role remains poorly understood. The present study compared the appearance of OSAD during both intramembranous and endochondral ossification and correlated it with early stages of mineralization. Parietal bone of 17,18 and 21 day-old embryos and mandibular condyle of 30 day-old Wistar rats were removed. Some specimens were frozen in liquid nitrogen while others were fixed in 0.1% glutaraldehyde+4% formaldehyde under microwave (MW) irradiation. Some specimens were left undecalcified, while others were decalcified in 4.13% EDTA, dehydrated in graded concentrations of ethanol and embedded in LR White resin. The expression of OSAD was analyzed by immunocy- tochemistry and Western blotting. Ultrathin sections were incubated with a polyclonal rabbit antibody against rat OSAD. In both intramembranous and endochondral ossification the gold particles were always uniformly distributed in the cytoplasm of osteoblasts but they only appeared in the mineralizing matrix when the fibrilar stage was taking place, remaining as a component of the mineralized bone matrix. Western blots carried out with the same antibody revealed that 17-day-old embryos contained slightly less OSAD than 18-day-old embryos, while immunoreactivity was weak in 21 day-old embryos. The results suggest that OSAD play a role in collagen fibril mineralization maybe as an organizer of matrix assembly or by retaining the mineral crystals into the matrix, besides exerting binding activities among bone matrix components. This article is part of a Special Issue entitled ECTS 2012. Disclosure of interest: None declared. doi:10.1016/j.bone.2012.02.343 PP155 Beneficial effects of long-term alpha-ketoglutarate (AKG) administration on tibia properties in domestic geese D. Niedziela a, ⁎, M.R. Tatara a , W. Krupski b , B. Tymczyna c , I. Luszczewska-Sierakowska d a Department of Animal Physiology, University of Life Sciences, Lublin, Poland b II Department of Radiology, Lublin, Poland c Departament of Conservative Dentistry, Medical University of Lublin, Lublin, Poland d Department of Animal Anatomy, University of Life Sciences, Lublin, Poland Abstract: Alpha-ketoglutarate (AKG) is a natural bioactive substance positively influencing bone tissue metabolism. The aim of the study was to investigate the effects of oral administration with AKG on skeletal system properties in geese during growth. Twenty male turkeys were randomly divided into 2 weight-matched groups (N=10 per group) at the age of 28 days of life. The first group consisted of control birds that received placebo orally. The second group (AKG group) received oral administration with AKG (0.4 g/kg b.w./d). The birds were kept under standard rearing conditions and slaughtered at the age of 108 days of life to analyse left tibia in terms of bone morphology (weight (W), length (L), cross-sectional area (A), second moment of inertia (Ix), mean relative wall thickness (MRWT), cortical index (CI)), volumetric bone mineral density (vBMD), as well mechanical properties (maximum elastic strength (Wy) and ultimate strength (Wf)). Using computed tomography technique (Somatom Emotion, Siemens), vBMD of the trabecular and the cortical bone of tibia was measured. Mechanical properties of tibia were estimated using three-point bending test and an INSTRON 3367 apparatus (Instron, USA). Statistical analysis was performed with a use of Student t-test and P b 0.05 was considered as statistically significant. Obtained results showed significantly increased weight, length, Ix, trabecular bone mineral density, cortical bone mineral density and Wy of tibia by 14.4%, 6.2%, 23.9%, 6.1%, 8.7%, and 31.8%, respectively, when compared to the controls (all P b 0.05). All these results indicate that long-term oral administration with AKG in geese improves vBMD of the trabecular and cortical bone compartments as well as morphological and mechanical properties of tibia in growing geese. Thus, the study have shown for the first time that AKG administration may be considered as an effective nutrient improving skeletal system quality in geese. This article is part of a Special Issue entitled ECTS 2012. Disclosure of interest: None declared. doi:10.1016/j.bone.2012.02.344 PP156 Alfacalcidols effect to shoulder fracture consolidation period at the patient with osteoporosis E. Solod ⁎ , A. Lazarev, S. Rodionova Central Institute of Traumatology and Orthopedics, Moscow, Russian Federation Abstract: Unstable fractures (classified Neer CS, 1970) in patients with systemic osteoporosis are characterized by the highest incidence of longer seam. Objective: Comparative assessment of the impact of alfacalcidols in the treatment of humerus fractures. Materials and methods: The study included 62 patients (mean age 69.7 years) with the proximal humerus fractures and a confirmed diagnosis of osteoporosis (senile and postmenopausal). All patients completed the operation, closed nailing tight V-spoke. The study group (48 patients) was receiving a daily alfacalcidol dose of 0.75–1.00 μg and 1000 mg of calcium carbonate in the post- operative period, the control group (14 patients) — only calcium carbonate. Patients were observed for 1 year. To control the formation of a full callus in the area surgical intervention and dynamics of the BMD at the spine (L1–L4) were performed at 6 and 12 months after surgery. Results: In the group receiving alfacalcidol the fracture was consolidated for 138 days and the group receiving only calcium carbonate — 188 days (p b 0.05). After 12 months BMD at the spine (L1–L4) in the study group was increased with an average of 2%. At the control group, BMD decreases about 2.5%. At the control group only 3 patients had a new compression fracture of the vertebral body. Conclusion: Alfacalcidols use in the postoperative period after minimally invasive osteosynthesis V-spoke shortens the formation of a full callus and prevents the progression of bone loss in the axial skeleton. This article is part of a Special Issue entitled ECTS 2012. Disclosure of interest: None Declared. doi:10.1016/j.bone.2012.02.345 PP157 Structural evaluation of the bone formed during endochondral ossification of young rats treated with bisphosphonates E. Rezende ⁎ , L.M.B. Ambrósio, V. Bradaschia-Correa, V.E. Arana-Chavez Oral Biology, University of São Paulo, São Paulo, Brazil Abstract: The bisphosphonates (BPs) are drugs with well known capacity of inhibiting bone resorption by osteoclasts. There are two major types of BPs, the nitrogenated and the non-nitrogenated, which possess different mechanisms of inhibition of the resorptive activity. The clastic cells are necessary, during the endochondral ossification, for the resorption of calcified cartilage septa and allow the invasion of osteogenic cells that secrete bone matrix over the calcified cartilage matrix. Although it has been reported that BP treatment promotes the enlargement of the cartilage zones, the effects of the both BP types on the bone formed during the endochondral ossification of long bones are not completely elucidated. The present Abstracts S112