ACADEMIA Letters National Molecular Surveillance of Recoveries/Mortalities Rate for COVID-19 in Pakistan-During First and Second Waves of Disease Adil Kalam Ayesha Sadiqa Javeria Samad Muhammad Usman Abstract The novel coronavirus disease (COVID-19) outbreak has caused signifcant global issues and challenged healthcare systems across the globe and in Pakistan as well. This infectious disease is found to be a pandemic and has massively impacted countries across the globe. The disease is still rapidly spreading and has caused many deaths in Pakistan, according to the world health organization; the Pakistan has ranked among the top 10 countries in the world reporting the highest number of new cases of COVID-19 per day during the frst wave of the outbreak. The second wave of COVID-19 is relatively high on the frst wave of disease in the current scenario of the pandemic in Pakistan. In Pakistan, the molecular technique real-time PCR is being applied for the qualitative confrmation of COVID-19 in the human nasopharyngeal swab samples according to WHO guidelines. Followed the extraction of COVID-19 nucleic acid, a probe-based real-time PCR assay is used for the detection of virus, mostly in all regions of country. The total of 416,499 confrmed cases are reported with the 2.0% death rate from Feb. to Dec.2020 in the frst and second waves of disease. Academia Letters, July 2021 Corresponding Author: Adil Kalam, adil.kalam@aku.edu Citation: Kalam, A., Sadiqa, A., Samad, J., Usman, M. (2021). National Molecular Surveillance of Recoveries/Mortalities Rate for COVID-19 in Pakistan-During First and Second Waves of Disease. Academia Letters, Article 1875. https://doi.org/10.20935/AL1875. 1 ©2021 by the authors — Open Access — Distributed under CC BY 4.0