J Appl Ichthyol. 2017;1–3. wileyonlinelibrary.com/journal/jai | 1 © 2017 Blackwell Verlag GmbH Received: 1 December 2016 | Accepted: 28 March 2017 DOI: 10.1111/jai.13413 SHORT COMMUNICATION New records of Speleogobius trigloides Zander & Jelinek, 1976 and S. llorisi Kovačić, Ordines & Schliewen, 2016 (Osteichthyes: Gobiidae) in the Aegean Sea S. Engin | A. Ç. Oruç | D. Seyhan | E. Irmak Faculty of Fisheries, Izmir Katip Celebi University, Izmir, Turkey Correspondence Semih Engin, Faculty of Fisheries, Izmir Katip Celebi University, Izmir, Turkey. Email: engin.semih@gmail.com 1 | INTRODUCTION The family Gobiidae is the largest fish family in the Turkish ma- rine waters with 48 species (9.2% of the total marine ichthyofauna) (Bilecenoğlu, Kaya, Cihangir, & Çiçek, 2014; Engin, Irmak, Seyhan, Akdemir, & Keskin, 2016). Recently, 33 gobiid fish species were veri- fied in the north-eastern area of the Aegean Sea (Engin et al., 2016). The genus Speleogobius is represented by two species: Speleogobius trigloides Zander & Jelinek, 1976 and the recently described Speleogobius llorisi Kovačić, Ordines and Schliewen, 2016. The pres- ent study represents new information on the range extension and morphological features of the Speleogobius species reported from the Aegean Sea. 2 | MATERIAL AND METHODS The underwater surveys were conducted with two closed-circuit rebreather (eCCR) divers. One female specimen of S. trigloides sam- pled from Melina Island/Ayvalık (39°20′08.34″N; 26°33′36.73″E; Figure 1) at 33 m depth in June 2016 and one male specimen of S. llorisi sampled from Mardaliç Island (38°54′39.67″N; 26°49′28.50″E; Figure 1) (Çandarlı) at 43 m depth in July 2016. The S. trigloides was captured barehanded and fixed 70% alcohol; the S. llorisi was sampled by hand net and fixed in 4% formaldehyde solution. Both specimens were deposited in the Marine Life Collection Centre of İzmir Katip Celebi University, Turkey (IKC.PIS.1090-91). The specimens were identified according to Miller (1986) and Kovačić, Ordines, and Schliewen (2016). Terminology of the lateral line system followed Sanzo (1911), Miller (1986), and Kovačić et al. (2016). Specimen measurements were taken under a stereomicroscope using a digital calliper point-to-point (accuracy of 0.01 mm) within 2 days after fixation. Scales in lateral series were counted from the axilla along the lateral midline, including scales over the origin of C. In the counts D2 and A, the last bifid ray was counted as one. Body depth was measured at anal fin origin, caudal peduncle length measured at end of A base to origin of C, head length measured from snout to mid- line opposite upper origin of opercle, head width measured between upper origin of post opercle, head depth measured between upper origin of posterior part of eyes. 3 | RESULTS Speleogobius specimens from the Aegean Sea were distinguishable from other Gobiidae families in the Mediterranean Sea: pelvic fins united into a disc; anterior membrane absent and posteriorly emargin- ated; suborbital sensory papillae row present; posterior oculascapular head canal absent; predorsal area scaled. Speleogobius trigloides and S. llorisi are separated from each other by several morphological characters: preopercular canal absent vs. preopercular canal present with pores γ, δ, ε; short snout vs. long snout; upper lip slightly protruding over lower lip vs. lower lip slightly protruding over upper lip; scale in transversal series 6 vs. 7. Detailed morphometric measurements are given in Table 1. First dorsal S. tri- gloides fin has red-brown spots. There are brownish and vertical bands on the trunk of S. llorisi. The grotto goby S. trigloides has a moderately-elongated body and a slender caudal peduncle. Head acute. Snout short, upper lip slightly protruding over lower lip. Large eyes. Predorsal area covered with seven rows of cycloid scales. Preopercular head canal absent. Lively coloration. General body reddish, with five pale saddles on the dorsal part of the trunk and eight pale blotches on the ventral part. Caudal fin transparent-yellowish, dorsal fins redish-brown with violet