Arch Vet Sci Med 2020; 3 (1): 31-39 DOI: 10.26502/avsm.013 Archives of Veterinary Science and Medicine 31 Research Article In-Vitro Antibacterial Activity of Probiotic Against Human Multidrug Resistant Pathogens Bhuvan Saud ⃰ , Pratima Pandey, Govinda Paudel, Gunaraj Dhungana, Vikram Shrestha Department of Medical Laboratory Technology, Janamaitri Foundation Institute of Health Sciences (JFIHS), Hattiban, Lalitpur, Nepal ⃰ Corresponding Author: Bhuvan Saud, Department of Medical Laboratory Technology, Janamaitri Foundation Institute of Health Sciences (JFIHS), Hattiban, Lalitpur, Nepal, Tel:+977-9848724226; E-mail: link2bhuvan@gmail.com Received: 20 November 2019; Accepted: 31 December 2019; Published: 15 February 2020 Citation: Bhuvan Saud, Pratima Pandey, Govinda Paudel, Gunaraj Dhungana, Vikram Shrestha. In-vitro Antibacterial Activity of Probiotic against Human Multidrug Resistant Pathogens. Archives of Veterinary Science and Medicine 3 (2020): 31-39. Abstract Objective: Probiotics have tremendous role in human health for disease prevention and treatment in both communicable and non-communicable diseases. The aim of study is to isolate, characterize and evaluate the antibacterial effect of probiotic against Multi-drug Resistant (MDR) human bacterial pathogens. Methodology: Total of 20 dairy (10 milk and 10 curds) samples were collected from dairy shops of Bhaktapur, Nepal. Lactobacilli spp. were isolated in de Man, Rogosa and Sharpe (MRS) agar and broth. Physiological, biochemical and tolerance tests were performed for the identification of Lactobacilli. Cell Free Supernatant (CFS) was used to determine antibacterial activity against 10 MDR bacterial species in Mueller-Hinton Agar (MHA) in in-vitro condition. Result: Out of 20 dairy product samples, altogether 14 (4 milk and 10 curd) samples were positive for Lactobacilli spp. The antibacterial effect of CFS against MDR isolates varied. Escherichia coli, Salmonella paratyphi, Salmonella typhi, Pseudomonas spp., Staphylococcus aureus, Proteus spp., Acinetobacter, showed antibacterial effect and,