Int. J. Chem. Technol. 2020, 4 (2), 179-184 Erenler and co-workers DOI: http://dx.doi.org/10.32571/ijct.795629 E-ISSN: 2602-277X 179 International Journal of Chemistry and Technology http://dergipark.org.tr/ijct Research Article Quantitative analysis of phenolic compounds of commercial basil cultivars (Ocimum basilicum L.) by LC-TOF-MS and their antioxidant effects Nusret GENC 1 , Mahfuz ELMASTAŞ 2 , İsa TELCİ 3 , Ramazan ERENLER 1,* 1 Department of Chemistry, Faculty of Arts and Sciences, Tokat Gaziosmanpasa University, Tokat 60240, Turkey 2 Department of Biochemistry, Faculty of Pharmacy, University of Health Sciences, Istanbul, Turkey 3 Department of Field Crops, Faculty of Agricultural Sciences and Technologies, Isparta University of Applied Sciences, Isparta 32260, Turkey Received: 16 September 2020; Revised: 09 October 2020; Accepted: 13 October 2020 *Corresponding author e-mail: rerenler@gmail.com Citation: Genç, N.; Elmastaş, M.; Telci, İ .; Erenler, R. Int. J. Chem. Technol. 2020, 4 (2), 179-184. ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ ABSTRACT Basil (Ocimum basilicum L.), an aromatic and medicinal plant, is used for food and pharmaceutical purposes. In this work, quantitative analyses of phenolic compounds for commercial basil cultivars, which are Sweet (1), Purple (2), Lettuce (3), Brush (4) grown in Tokat city in Turkey's ecology, were executed by Liquid Chromatography Time-of-Flight Mass Spectrometry (LC-TOF-MS). Antioxidant activities of related genotypes were determined using 2,2-Diphenyl-1- picrylhydrazyl (DPPH)’s radical, 2,2′-Azino-bis(3- ethylbenzothiazoline-6-sulfonic acid (ABTS)’s diammonium salt and the ferric reducing antioxidant power (FRAP) assays. The activity-compound relationship was revealed. Brush (4) genotype revealed the most DPPH [296 µmol e TE (Trolox equivalent/g DW (gram dry weight)], ABTS (706 µmole TE/g DW), and FRAP (650 µmole TE/g DW) activities. It was determined that rosmarinic acid was in the highest amount in all genotypes. Among the genotypes, it was determined that Lettuce contained the most rosmarinic acid with a value of 180460.6 (mg kg -1 DW). Keywords: Ocimum basilicum L., antioxidant activity, basil cultivar, quantitative analysis, LC-TOF-MS. Ticari fesleğen çeşitlerinin (Ocimum basilicum L.) fenolik bileşiklerinin LC-TOF-MS ile kantitatif analizi ve antioksidan etkileri ÖZ Aromatik ve tıbbi bir bitki olan reyhan (Ocimum basilicum L.) gıda ve ilaç amaçlı kullanılmaktadır. Bu çalışmada, Türkiye'nin ekolojisinde Tokat şehrinde yetişen Sweet (1), Purple (2), Lettuce (3), Brush (4) olmak üzere dört ticari fesleğen çeşidinin fenolik bileşiklerinin kantitatif analizleri LC-TOF/MS ile gerçekleştirilmiştir. İlgili genotiplerin antioksidan aktiviteleri, 2,2-Difenil-1-pikrilhidrazil (DPPH) radikali, 2,2-azino-bis(3-etilbenzotiazolin-6-sülfonik asit (ABTS)’in diamonyum tuzu ve indirgenme gücü (FRAP) yöntemleri kullanılarak belirlendi. Aktivite-bileşik ilişkisi gösterildi. Brush 4 genotipi en yüksek DPPH [296 µmol TE (Trolox ekivalent) /g DW (gram kuru ağırlık)], ABTS (706 µmol TE/g DW) ve FRAP (650 µmol TE/g DW) aktivitesi gösterdi. Rosmarinik asidin bütün genotiplerde en yüksek miktarda olduğu belirlendi. Genotipler arasında Lettuce (3)’ün, 180460.6’ lik (mg kg -1 kuru bitki) bir değer ile en fazla rosmarinik asit içerdiği belirlendi. Anahtar Kelimeler: Reyhan, antioksidan aktivitesi, reyhan kültürü, kantitatif analiz, LC-TOF-MS. --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 1. INTRODUCTION Natural products have been consumed extensively for therapeutic properties for centuries. 1,2 Herbal medicines have been accepted as a main basis of crucial health care in many countries. 3 Almost 80% of the world population still depends on folk medicine. Spectroscopic developments have led to the isolation of active compounds from plants in the 19 th century. Hence, many organic advances have been inspired by natural products. Synthetic chemists have begun to synthesize natural products with pharmaceutical properties due to the isolation of natural products. 4 Natural compound frameworks have been regarded as