80 Journal of Dentistry Indonesia 2015, Vol. 22, No. 3, 80-84 doi:10.14693/jdi.v22i3.402 Bisphenol-A Release and Genotoxicity Differences of Three Lingual Retainer Adhesives Materials Lydia Winarta 1 , Rahmi Amtha 2 , Boedi O. Roeslan 3 , Himawan Halim 1 1 Postgraduate Program, Trisakti University, Jl. Kyai Tapa No.1, Jakarta, Indonesia 2 Department of Oral Medicine, Trisakti University, Jl. Kyai Tapa No.1, Jakarta, Indonesia 3 Department of Biochemistry, Trisakti University, Jl. Kyai Tapa No.1, Jakarta, Indonesia 4 Department of Orthodontics, Trisakti University, Jl. Kyai Tapa No.1, Jakarta, Indonesia Correspondence e-mail to: lydia_oey@yahoo.com ABSTRACT Objectives: The objective of the study were to determine if there was any (bisphenol A) BPA release from three adhesive brands, to determine the differences of BPA release between three adhesive brands, to determine the genotoxicity from three adhesive brands, and to determine the correlation of BPA release and genotoxicity. Methods: Three branded adhesives materials were polimerized in mold and immersed in pH 7 and 4 artifcial saliva from 24 to 720 hours. The artifcial saliva was tested with spectrophotometry test to see BPA release at 24, 240, 480, and 720 hours, then freeze dried to get solid extract. Combination of the extract and lymphocite culture (male and female) then tested with in vitro cytokinesis-block micronucleus (MN) assay to see genotoxicity level of three adhesives at 24, 240, 480, and 720 hours as well. Results: The BPA release occured at 720 hours by Adhesive 1: 0.013μg/L; Adhesive 2: 0.11 μg/L; Adhesive 3: 0.036μg/L. There was a statistically signifcant difference between BPA release with time (F = 505.98; p=0.00) and brands (F = 147.65; p = 0.00). Time and BPA release interaction also showed a statistically signifcant difference (F=13.35; p=0.00). Genotoxicity can be seen at 720 hours on Flowtain LV sample (MN frequency: male: 0.044; female: 0.053). Conclusion: The number of BPA release of all brand can be seen from the frst 24 hours, and were increasing from 24 to 720 hours. Genotoxicity can be seen from one of the adhesive brand at 720 hours.There was correlation between BPA leaching and micronucleus frequency. Keywords: BPA, spectrophotometry, micronucleus test ORIGINAL ARTICLE INTRODUCTION A lingual retainer is used to maintain tooth position post orthodontic treatment. It is bonded to the lingual side of anterior teeth using composite. Various composites have been described for use in this technique including both restorative and orthodontic bonding materials. Various restorative and orthodontic bonding materials have been used as lingual retainer materials. The fowable composites as bonding for lingual retainers are increasingly used. Several studies have tested the shear bond strength (SBS) of Filtek Supreme and Tetric Flow. 1 The SBS values for Filtek Supreme, and Tetric Flow were 22.4 ± 16.3, and 16.8 ± 11.8 MPa, respectively, and mean pullout values were 26.3 ± 9.4, and 33.8 ± 18.0 N, respectively. These fowable composites provided satisfactory SBS and wire pullout values, comparable to a standard orthodontic resin. Therefore, they can be used as an alternative for direct bonding of lingual retainers. 1 Bisphenol A (BPA) is a synthetic chemical with known disruptive endocrine and weak estrogenic properties. 16 lobal attention to its use as an environmental contaminant to human health is increasing. A great exposure to BPA could have an impact on endocrine-disrupting properties in human. 2 In clinical dentistry, BPA is a precursor of the bisphenol A glycidyl methacrylate (bis-GMA), bisphenol-A di-methacrylate (bis-DMA), and bisphenol A ethoxylated di-methacrylate (bis-