KnE Medicine ISGH4 The 4th International Seminar on Global Health Volume 2022 Research article Antioxidant Activities of Green Tea (Camellia Sinensis L.) Leaves From Ciwidey, West Java Arina Novilla*, Wikan Margahyani, and Dwi Davidson Rihibiha Department of Medical Laboratory Technology, Faculty of Health Science and Technology, Universitas Jenderal Achmad Yani Cimahi, Indonesia Abstract. It has been reported that polyphenols-rich diets provide some protection against the development of cancers, cardiovascular diseases, diabetes, osteoporosis and neurodegenerative diseases. Exogenous antioxidants are abudantly found in plants. Tea (Camellia sinensis L.; family Theaceae), which is the most popular beverage in Southeast Asia, is known for its medicinal properties. In this study, we evaluated antioxidant activity as well as phenol and flavonoid content in green tea leaves from PPTK Gambung Ciwidey in West Java. Antioxidant activity was observed through measuring 2,2- diphenyl-1 picrylhydrazyl (DPPH) scavenging activity. The total phenolic content was determined by the Folin Ciocalteu method. The green tea leaf extract showed the highest DPPH-scavenging activity at a concentration of 25 ug/ml (DPPH = 94.55%;IC 50 = 0.54 µg/mL). Furthermore, the total phenolic and flavonoid contents of the green tea leaf extract were 36.64 and 8.43 mg QE/mg extract, respectively. These findings showed that the green tea leaf extract exhibited antioxidant activity. Keywords: Camellia sinensis L, flavonoid, antioxidant 1. Introduction Free radicals are biochemical responses in the body which further promotes cancer, ischemic heart disease, inflammation, diabetes, aging, atherosclerosis, immunosuppres- sion, and neurodegenerative disorders. [1-3] Antioxidant system in human body is usually responsible to scavenge the radicals. However, this might be interferred by excessive reactive oxygen species (ROS) and reactive nitrogen species (RNS) due to the exposure of cigarette smoking, alcohol, radiation, or environmental toxins. [2, 4, 5] Exogenous antioxidants are considered to restore such event by inhibiting oxidative chain reaction [6]. The exogenous antioxidants are mostly derived from food and medicinal plants [7-10]. These natural antioxidants are mainly polyphenols (phenolic acids, flavonoids, anthocyanins, lignans and stilbenes), carotenoids (xanthophylls and carotenes) and vitamins (vitamin E and C) [6]. Polyphenols and carotenoids exhibit many biological How to cite this article: Arina Novilla*, Wikan Margahyani, and Dwi Davidson Rihibiha, (2022), “Antioxidant Activities of Green Tea (Camellia Sinensis L.) Leaves From Ciwidey, West Java” in The 4th International Seminar on Global Health, KnE Medicine, pages 143–150. DOI 10.18502/kme.v2i2.11077 Page 143 Corresponding Author: Arina Novilla; email: arin_novilla@yahoo.co.id Published: 3 June 2022 Publishing services provided by Knowledge E Arina Novilla et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. Selection and Peer-review under the responsibility of the ISGH4 Conference Committee.