How to Cite: Padugupati, S., Ramamoorthy, S., Thangavelu, K., Sarma, D. V. H. S., & Jamadar, D. (2022). Effect of berberine in comparison to metformin on the biophysical and biochemical parameters in diabetic albino Wistar rats. International Journal of Health Sciences, 6(S2), 4998–5014. https://doi.org/10.53730/ijhs.v6nS2.6256 International Journal of Health Sciences ISSN 2550-6978 E-ISSN 2550-696X © 2022. Manuscript submitted: 27 Feb 2022, Manuscript revised: 18 March 2022, Accepted for publication: 09 April 2022 4998 Effect of berberine in comparison to metformin on the biophysical and biochemical parameters in diabetic albino Wistar rats Suhasini Padugupati, Research Scholar Department of Biochemistry, Palamur Bio Sciences Pvt Ltd, in affiliation with Manipal Academy of Higher Education, Manipal, Karnataka, India Email: drduhasinimd@gmail.com S Ramamoorthy , Chief Executive Officer, Department of Toxicology, Palamur Bioscience Pvt.Ltd, Mahabubnagar, Telangana, India Email: ram.murthy@palamurbio.com Kumar Thangavelu , Director, Department of Toxicology, Palamur Bioscience Pvt.Ltd, Mahabubnagar, Telangana, India Email: kumar@palamurbio.com D V H S Sarma , Professor and Head, Department of Biochemistry, S.V.S Medical College, Mahabubnagar, Telangana, India Email: drdvhs.sarma123@gmail.com Deepak Jamadar Statistician Cum Assistant Professor, Department of Community Medicine, Palamur Bioscience Pvt.Ltd, Mahabubnagar, Telangana, India Email: deepak3march@gmail.com Abstract---Introduction: Diabetic endothelial dysfunction is accompanied by increased oxidative stress and upregulated proinflammatory and inflammatory mediators in the endothelial vasculature. Aim of this study is to investigate the effect of Berberine, a natural alkaloid, on the oxidative stress, inflammation and its anti- oxidant effect in streptozotocin diabetic rats and to compare the effectiveness of FF with that of Metformin (Met) Material & Methods: This experimental animal study was conducted at animal house. The sample size included 174 albino wistar rats divided into 3 Groups, one control groups (C) Diabetic and untreated and two test groups. T1 Diabetic and treated with metformin 75 mg/kgwt/day) and T2 (T –