J Environ Sci Public Health 2022;6 (2):189-203 DOI: 10.26502/jesph.96120166 Journal of Environmental Science and Public Health 189 Research Article Electric Cell-Substrate Impedance Sensing (ECIS) for Analyzing the Effect of Environmental Pollutants - A Study of Diesel Exhaust Nanoparticles Amalu Navas 1 , Maya Nandkumar A 2* 1 PhD Scholar, Division of Microbial Technology, BMT Wing, SCTIMST, Poojappura, Thiruvananthapuram, Kerala, India 2 Scientist ‘G’ and Head, Division of Microbial Technology, BMT Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram-12, Kerala, India * Corresponding Author: Dr A Maya Nandkumar, Scientist ‘G’ and Head, Division of Microbial Technology, BMT Wing, SCTIMST, Poojappura, Thiruvananthapuram, Kerala, India Received: 11 May 2022; Accepted: 18 May 2022; Published: 26 May 2022 Citation: Amalu Navas, Maya Nandkumar A. Electric Cell-Substrate Impedance Sensing (ECIS) for Analyzing the Effect of Environmental Pollutants - A Study of Diesel Exhaust Nanoparticles. Journal of Environmental Science and Public Health 6 (2022): 189-203. Abstract ECIS is a morphological biosensor that records the electrical properties of cell-covered microelectrodes in an AC circuit, including impedance (ohm), resistance (ohm), and capacitance (μFarad). The objective of the current study was to analyze the suitability of ECIS as a label-free in vitro assay system to understand the effect of external stimuli on cells in real-time, vis-à-vis regular endpoint assays of cytotoxicity. The study analyzed whether fluctuations in the electrical properties of cell-covered microelectrodes reflected dynamic changes in cell morphology on exposure to diesel exhaust particles. Exposure of A549 monolayers in 8 well microarrays to DEP caused significant changes in microelectrode resistance (ohm @4 kHz) as compared to controls within 24 h. Variations in impedance were found to have a dose-dependent effect. We compared our results from ECIS with classic cytotoxicity analyses