Mostafa M. Abo Elsoud et al., J Inv. Bio. Pharm. Sci. 2020; 5(3): 18-26 OėĎČĎēĆđ AėęĎĈđĊ JĔĚėēĆđ Ĕċ IēěĊēęĎĔēĘ Ďē BĎĔĒĊĉĎĈĆđ Ćēĉ PčĆėĒĆĈĊĚęĎĈĆđ SĈĎĊēĈĊĘ Published by ScienzTech Publication Journal Home Page: www.scienztech.org/jibps Production of α-amylase(s) by Aspergillus ϔlavus, F7 attacking water hyacinth ground preparation (WHGP) under solid state fermentation Eman I. El-Tabakh 1 , Mostafa M. Abo Elsoud *2 , Marwa S. Salem 1 , Nagwa M. Sidkey 1 1 Department of Botany and Microbiology, Faculty of Science, Al-Azhar University (Girls Branch), Cairo, Egypt 2 Department of Microbial Biotechnology, National Research Centre, Egypt Article History: Received on: 19 Jul 2020 Revised on: 31 Jul 2020 Accepted on: 09 Aug 2020 Keywords: α-amylase, Aspergillus ϐlavus, F7, production, environmental and nutritional, water hyacinth AćĘęėĆĈę Some environmental and nutritional parameters controlling the biosynthesis of α-amylase from Aspergillus ϔlavus, F7 attacking the water hyacinth have been investigated under solid state fermentation conditions for maximum amylase production. The following optima’s were recorded for the highest α- amylase yield; Incubation period 7 days; temperature, 30 o C; pH, 5; inoculum size, 3X10 8 spores/ml; ϐlask volume 100 ml capacity; hyacinth fresh weight 5 g; tap water, 25 ml. Under these conditions, starch showed remarkable stim- ulatory effect; nitrogen sources and amino acids have no stimulatory effect. Pyridoxal hydrochloride, B6 at a concentration of 200 ppm exhibited a stimu- latory effect on biosynthesis of α-amylase. * Corresponding Author Name: Mostafa M. Abo Elsoud Phone: Email: masnrc@gmail.com ISSN: 2456-818X DOI: https://doi.org/10.26452/jibps.v5i3.1439 Production and Hosted by ScienzTech.org © 2020 | All rights reserved. INTRODUCTION Water hyacinth (Eichhornia crassipes) is an intruder ϐloating water weed that has spread throughout freshwater bodies. Egypt has the oldest record of water hyacinth in Africa since the late 1800s. The weed prohibit electricity generation, clog irrigation canals, hindering ϐish production; increases water loss resulting from evaporation and block light from penetrating river water; and facilitates propagation of bilharzia and malaria diseases. Since 1991 Egypt has depended exclusively on mechanical harvesting method to control water hyacinth [1]. This plant contains a high proportion of protein (49.6%), total lipids (16.0%), total carbohydrates (26.9%), ϐibre (1.7%) and 5.8% ash. In addi- tion, calcium, magnesium, copper, zinc, manganese and potassium were determined [2]. Recently, [3] reported elements of water hyacinth are C, O, N, Na, Mg, Al, Zr, Cl, K, Ca, Si, Ti, and Fe revealing domi- nant elements. Many trials were carried out to uti- lize water hyacinth as a sole carbon source and a fer- mentable substrate for fungal production of extra- cellular enzymes were reported [4–7] in addition production of biogas [8], bioethanol [9, 10] were reported. One of the objectives of the biotechnology is the utilization of wastes from environment [11, 12], agriculture [13, 14], food industry [15–18] indus- trial [19, 20] for the production of enzymes, pro- teins, animal feed, and energy and at the same time combating the pollution of the environment. Waste disposal involves using appropriate microorganism to decompose organic wastes which are too haz- ardous to treat by other means [21]. Amylases are crucial enzymes which hydrolyze gly- cosidic linkages in starch and produce as primary products dextrins and oligosaccharides. They are 18 © ScienzTech Publication | Journal of Inventions in Biomedical and Pharmaceutical Sciences