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doi: 10.1002/dta.2179
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RNA stabilized blood tubes to measure haemoglobin
concentration
Nicolas Leuenberger
1*
, Céline Schobinger
1
, Olivier Salamin
2
, Martial Saugy
2
and Tiia
Kuuranne
1
1
Swiss Laboratory for Doping Analyses, University Centre of Legal Medicine, Lausanne and
Geneva , Centre Hospitalier Universitaire Vaudois and University of Lausanne, Switzerland
2
Center of Research and Expertise in anti-Doping sciences - REDs, University of Lausanne,
Switzerland
Corresponding Author for correspondence and reprint requests:
Nicolas Leuenberger, PhD
Swiss Laboratory for Doping Analyses
Ch. Des Croisettes 22
1066 Epalinges
Email: Nicolas.leuenberger@chuv.ch
Phone: +41 21 314 70 94
Fax : +41 21 314 70 95
Abstract
Recent investigation demonstrated that measurements of reticulocytes gene transcripts could
serve as potential biomarkers to detect blood doping. Reticulocytes gene transcripts were
collected into stabilized RNA blood tubes that allowed gene transcripts stability up to five
days at room temperature. In order to investigate applicability of the equipment to the routine
sample collection, measurement of another parameter which is used to detect blood doping,
namely haemoglobin concentration measurement was tested in RNA stabilized blood tubes.
A strong correlation between paired EDTA and RNA stabilized blood samples was observed.
In addition, storage stability was investigated.