Int. J. Pharm. Sci. Rev. Res., 65(2), November - December 2020; Article No. 09, Pages: 50-55 ISSN 0976 – 044X International Journal of Pharmaceutical Sciences Review and Research Available online at www.globalresearchonline.net ©Copyright protected. Unauthorised republication, reproduction, distribution, dissemination and copying of this document in whole or in part is strictly prohibited. 50 Suvarna S. Vadje 1 , Rajendra K. Surawase 2 , Santosh S. Surana 3 Department of Pharmaceutics; Loknete Dr. J. D. Pawar College of Pharmacy Manur, Kalwan, Maharashtra, 423501, India. *Corresponding author’s E-mail: Suvarnavadje259@gmail.com Received: 18-09-2020; Revised: 21-11-2020; Accepted: 29-11-2020; Published on: 15-12-2020. ABSTRACT Formulation of poorly water-soluble drug has always been a challenging problem confronted by the formulation scientist since more than the 40% of the new chemical entities being generated through the drug discovery programme are poorly water soluble. A poorly water-soluble drug indicates insufficient bioavailability. The purpose of this study was to formulate and evaluate the furosemide nanosuspension by nanoprecipitation method with the different stabilizer like Poloxamer 188, PVP K30 and tween 80. The mixture of the drug and methanol as an organic phase and distilled water containing carrier as an aqueous phase. Nanosuspension containing Poloxamer 188 (30mg) and tween 80 (10mg) were selected to be the best based on their drug content, entrapment efficiency and particle size. The result showed that the prepared nanosuspension have particle size in the range of 0.0035 to 0.017µm. The nanosuspension of furosemide was successfully prepared using nanoprecipitation method. Keywords: Furosemide, Nanosuspension, Nanoprecipitation, Poloxamer 188. QUICK RESPONSE CODE → DOI: 10.47583/ijpsrr.2020.v65i02.009 DOI link: http://dx.doi.org/10.47583/ijpsrr.2020.v65i02.009 INTRODUCTION ral route of drug delivery is the most commonly used route because of its convenience, painless administration and patient compliance than other routes of drug administration. 1 More than 40% of the NCEs generated through the drug discovery programme are poorly water soluble. Therefore the pharmaceutical industries seeking new approach in order to obtain an adequate oral bioavailability of this type of new chemical entities. Formulation of poorly water soluble or practically insoluble (i.e. BCS class II and BCS class IV drug) drug has always been a challenging problem confronted by the pharmaceutical scientist. 2 There are various approaches have been used to solve the problems of poor solubility and bioavailability such as prodrug, salt formation, complexation (inclusion complexation with β- Cyclodextrin), Co-solvency, use of surfactant, liposomes, microemulsion etc., but these methods have the limitations such as requirement of the large amount of the additives induce the stability and toxicity issues. 3 These methods lack the universal applicability to all drug. 3,4 Nanotechnology can be used to solve the problems associated with the earlier approaches to enhance solubility and bioavailability. 5,6 Reduction of the particle size to nanoscale can be applicable to both BCS class II and IV to increase the solubility, dissolution and hence their absorption into the gastrointestinal tract. 7 Nanotechnology have various advantages over other conventional technology such as it can enhanced solubility, dissolvability, extends the oral bioavailability, lessens the amount of dose. There are various type of drug delivery system using the nanotechnology such as nanosuspension, solid lipid nanocrystals, nanoemulsion, etc. 8,9 Nanosuspension preparation is technically easy, simple and less costly than other nanosizing method. 10 Nanosuspension is the colloidal dispersion of the drug particle in an aqueous vehicle mainly water which is stabilized by the surfactant, polymer or mixture of the both, for either oral, topical use or the parenteral and the pulmonary administration, with the reduced particle size which leads to increase the dissolution rate and improved bioavailability. 11,12 The particle size distribution of the solid drug particle in nanosuspension is usually less than 1 micron with an average particle size range in between 200- 600nm. 13 Nanosuspension technology is most suitable for the compound with high log P value, high melting point and high dose. 14-16 There are mainly two types of stabilizer used for the stabilization of nanosuspension – steric and electrostatic stabilizer. 16 No single one stabilizer suitable for all drug nanosuspension. 17 In the nanosuspension drug particle size reduction leads to an increase into the particle surface area and consequently the rate of dissolution as described by the Nernst – Brunner and Levich modification of the Noyes- Whitney equation. Increase in saturation solubility postulated by the particle size reduction due to increase into the dissolution pressure explained by Ostwald – Freundlich equation. 11, 18 The Nanosuspension can be prepared by bottom- up technology, top- down and third one is the combination technology. The ‘Bottom up Formulation and Evaluation of Nanosuspension Drug Delivery System of Furosemide Produced by Nanoprecipitation Method O Research Article