This article is protected by copyright. All rights reserved 1 Research article Multiplex RT-PCR for rapid detection of viruses commonly causing diarrhea in pediatric patients † Aksara Thongprachum a,b , Pattara Khamrin c , Ngan Thi Kim Pham a , Sayaka Takanashi b , Shoko Okitsu a,b , Hiroyuki Shimizu d , Niwat Maneekarn c , Satoshi Hayakawa a , Hiroshi Ushijima a,b * a Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan b Department of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan c Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand d Department of Virology II, National Institute of Infectious Disease, Tokyo, Japan *Correspondence: Hiroshi Ushijima, M.D., Ph.D. Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine 30-1 Oyaguchi Kamicho, Itabashi-ku, Tokyo, 173-8610, Japan E-mail: ushijima-hiroshi@jcom.home.ne.jp Phone: +81-3-3972-8111 (Ext 2263); Fax: +81-3-3972-9560 Running title: Multiplex RT-PCR for rapid detection of viruses causing diarrhea † This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi: [10.1002/jmv.24711] Received 28 July 2016; Revised 5 October 2016; Accepted 7 October 2016 Journal of Medical Virology This article is protected by copyright. All rights reserved DOI 10.1002/jmv.24711 Accepted Article