1 Protein Kinase C Mediates Lysophosphatidic Acid - Induced NF- B Activation and Interleukin-8 Secretion in Human Bronchial Epithelial Cells Rhett Cummings 1 , Yutong Zhao 1 , David Jacoby 2 , E. William Spannhake 3 , Motoi Ohba 4 , Joe G.N. Garcia 1 , Tonya Watkins 1 , Donghong He 1 , Bahman Saatian 1 and Viswanathan Natarajan 1# 1 Division of Pulmonary and Critical Care Medicine and Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, 2 Department of Medicine, Oregon Health and Science University, Portland, OR , 3 Department of Environmental Health Sciences, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD and 4 Institute of Molecular Oncology, Showa University, Tokyo, Japan. # Address Correspondence to: Dr. Viswanathan Natarajan, Ph.D. Department of Medicine Division of Pulmonary and Critical Care Medicine MFL 675, Center Tower 5200, Eastern Avenue Baltimore, MD 21224 Tel. 410-550-7748; Fax. 410-550-8571; email. vnataraj@jhmi.edu Short Title: Protein Kinase C in LPA-induced NF- B activation and IL-8 secretion. Key words: Lysophosphatidic acid; Interleukin-8; NF B; Protein Kinase C ; neutrophils infiltration; human bronchial epithelial cells. The abbreviations used are: BAL, bronchoalveolar lavage; BEAS-2B, human bronchial epithelial cell line; ECL, enhanced chemiluminescence; EDG, endothelial differentiation gene; EMSA, electrophoretic mobility shift assay; FBS, fetal bovine serum; HBEpCs, human bronchial epithelial cells; HBSS, hanks balanced salt solution; GAPDH, glyceraldehyde phosphate dehydrogenase; HUVEC, human umbilical vein endothelial cell; IL-8, interleukin-8; I B, inhibitory kappa B; LPA, lysophosphatidic acid; MIP-2, macrophage inhibitory protein-2; MOI, multiplicity of infection; NF- B, nuclear factor kappa B; PDGF, platelet derived growth factor; PKC, protein kinase C; PLD, phospholipase D; PT X , pertussis toxin; S1P, sphingosine-1-phosphate; TNF- , tumor necrosis factor- . JBC Papers in Press. Published on July 26, 2004 as Manuscript M404045200 Copyright 2004 by The American Society for Biochemistry and Molecular Biology, Inc. by guest on November 17, 2017 http://www.jbc.org/ Downloaded from