_____________________________________________________________________________________________________ *Corresponding author: Email: ifeoluwasylva@gmail.com; International Journal of TROPICAL DISEASE & Health 43(6): 1-11, 2022; Article no.IJTDH.85492 ISSN: 2278–1005, NLM ID: 101632866 Assessment of Knowledge, Attitude and Practices of Hand Hygiene among Medical and Nursing Students Ifeoluwa Silvanus Abiodun a* , Esther Olufunke Monday a , Chizoba Jessica Aneke a , Rukayat Akinola-Oloyede a , Olawale Omotayo Olatunji a and Abiola Abigael Oyindamola a a All Saints University School of Medicine, Roseau, Dominica. Authors’ contributions This work was carried out in collaboration among all authors. All authors read and approved the final manuscript. Article Information DOI: 10.9734/IJTDH/2022/v43i630594 Open Peer Review History: This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://www.sdiarticle5.com/review-history/85492 Received 24 January 2022 Accepted 01 April 2022 Published 09 April 2022 ABSTRACT Aims: This study aims to determine and compare the levels of handwashing knowledge, attitude and practice among medical and nursing students of All Saints University School of Medicine, Dominica. Study Design: This was a quantitative, cross-sectional study. Place and Duration of Study: All Saints University School of Medicine, Dominica between May and August, 2021. Methodology: A total of 73 students (26 MD students, 18 Clinical students, 22 Nursing students and 7 Pre-med students) of All Saints University Dominica participated in the study, after their informed consent was obtained. The study data was collected from registered medical and nursing students of All Saints University, Dominica. This involved the use of a self-designed, structured questionnaire that was electronically distributed to students and analyzed electronically using SPSS version 21. Results: Though all participants admitted having heard of hand hygiene practices, all nursing students (100%) affirmed having sufficient knowledge about hand hygiene while 28.6% of the pre- med students participating did not feel they had sufficient knowledge (P<0.05). Also, a large percentage of the respondents that were nursing students (90.9) claimed to have had a formal training in hand hygiene. This was closely followed by clinical students (72.2%) while MD and pre- med students only had 38.5% and 28.6% respectively. Original Research Article