Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2012, 4(6):3020-3030 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 3020 Aluminum and the effects of chelating agents on Muscle, Kidney and Liver of cirrhinus mrigala S. Sivakumar, Chandra Prasad Khatiwada and J. Sivasubramanian Department of Physics, Annamalai University, Annamalainagar, Tamilnadu _____________________________________________________________________________________________ ABSTRACT The study of biological indicator organism is more important than analyzing water or sediments for monitoring heavy metal pollution in the aquatic environment .Non-essential elements enter the animals and accumulate to the different organs so that chelating agents are most versatile and effective antidotes to eliminate the metals toxicities. The aim of our present study is to finds out bioaccumulations of aluminum and the effects of chelating agents DFO and DFP in Muscle, kidney and liver tissues of Cirrhinus mrigala by using Inductively Coupled Atomic Emission Spectrometer (ICP-AES). This study finds out the accumulation of aluminum is Muscle>Kidney>Liver. The present result suggests that DFO and DFP reduce the aluminum concentration in the tissues of Cirrhinus mrigala fingerlings and both are efficient chelator. Aluminum toxicity is a wide spread problem in all forms of life, including humans, animals, fishes, plants, and cause wide spread degradation of the environment and health. Keywords: Bioaccumulation, aluminum, chelating agents, ICP-AES _____________________________________________________________________________________________ INTRODUCTION The estimation of aluminium concentration in the samples of fish had made using an ICP-AES (ISA JOBIN YVON 24 MODAL) and the analytical standard was prepared from the aluminium stock solution. Metal contamination from laboratory was avoided and triplicates of each sample were analysed. The metal concentration in different tissue samples were calculated as follows [1], [2]. Metal concentration (μg/g) = [ICP-AES reading (μg/ml)/sample mass (μg)]x samples volume(ml). Bio concentration of chemicals by aquatic biota is an important factor in the assessment of the potential hazard of chemicals to the environment. The bio concentration factor or Biological Magnification Factor (BMF) is calculated as BMF = K 1 /K 2 =Chemical concentration in each part of the fish (μg/g wet weight)/ Chemical concentration in water (μg/l). Calculation of excretion rate constant chemicals from the whole fish body/organ is C = C 0 e -k2t Where, C = Chemical concentration in whole body/ organ (μg/g wet weight) at time t. C 0 = Initial chemical concentration in whole fish body/ organ (μg/g wet weight). K 2 = Excretion rate constant (h -1 ) and t= time (h -1 ). Aluminium, which is the most abundant metal and comprises about 8% of the Earth's crust, is found in combination with oxygen, silicon, fluorine and other elements in the soil, rocks, clays and gems [3]. It has no known biological function [4]. Presently, aluminum utensils are widely used throughout the world, especially in developing countries [5]. The use of such tools may increase an individual’s aluminium exposure, particularly when these are used with salty, acidic or alkaline foods [6]. Additionally, aluminium and its salts are commonly used in daily life as it is