[VOLUME 5 I ISSUE 4 I OCT. – DEC. 2018] e ISSN 2348 –1269, Print ISSN 2349-5138 http://ijrar.com/ Cosmos Impact Factor 4.236 Research Paper IJRAR- International Journal of Research and Analytical Reviews 599 “In vitro studies for anti-fungal activity of Homoeopathic medicines against plant fungus Gibberella fujikuroi .” Snehal Ashok Barkund 1 , Pashmin Kaur Anand 2 , Mayur Arjun Aswani 2 , Arun Bhargav Jadhav 3 , Bipinraj Nirichan Kunchiraman 4 & Chetan Hanamantrao Shinde 5 . 1 Post - Graduate Scholar, Department of Homoeopathic Pharmacy, Bharati Vidyapeeth (Deemed to be) University, Homoeopathic Medical College & Hospital, Katraj – Dhankawadi, Pune, India. 2 Department of Microbiology, Bharati Vidyapeeth (Deemed to be) University, Rajiv Gandhi Institute of Information Technology and Biotechnology, Katraj – Dhankawadi, Pune, India. 3 Principal, Bharati Vidyapeeth (Deemed to be) University, Homoeopathic Medical College and Hospital, Katraj - Dhankawadi, Pune, India. 4 Assistant Professor, Department of Microbiology, Bharati Vidyapeeth (Deemed to be) University, Rajiv Gandhi Institute of Information Technology and Biotechnology, Katraj – Dhankawadi, Pune, India. 5 Associate Professor, Department of Homoeopathic Pharmacy, Bharati Vidyapeeth (Deemed to be) University, Homoeopathic Medical College & Hospital, Katraj – Dhankawadi, Pune, India. Received: July 02, 2018 Accepted: August 24, 2018 ABSTRACT Gibberella fujikuroi is a plant pathogen which affects rice and other plants and causes Bakanae disease. In this anti-fungal study author aimed to evaluate the in vitro effects of Homoeopathic Medicines Thuja occidentalis, Silicea, Mercurius Solubilis, Lachesis Mutus, Calcarea Carbonica in (6C, 12C, 30C, 200C, 1M, 10M, CM) potencies against Gibberella fujikuroi. The Fungus was screened by agar well diffusion method and MIC according to percentage method. It was found that Thuja occidentalis (10M), Silicea (200C and 1M), Mercurius Solubilis (6C, 12C and 10M), Lachesis Mutus (6C and 200C), Calcarea Carbonica (30C and 200C) inhibits 100% growth of Gibberella fujikuroi. These Homoeopathic Medicines can be used as alternative agro-homoeopathic fungicides in the agriculture field. Keywords: Anti-fungal, Homoeopathic Medicines, Gibberella fujikuroi, MIC, Potato Dextrose Broth (PDB). Introduction – Gibberella fujikuroi is a plant pathogen fungus which produces gibberellins and secondary metabolites such as carotenoids, bikaverin, fusarin, phytotoxins and mycotoxins. [1,2] This fungus has majorly affected plants, humans and animals. Gibberella fujikuroi is a spore killer fungus. When one parent carries a killer element (SK k ) and another carries the sensitive counterpart (SK S ) causes killing. [3] Gibberella fujikuroi complex (GFC) has more than 50 distinct species or phylogenetic lineages. In this 34 species are described by their morphological characters, 10 species on the basis of sexual fertility and 20 species produces mycotoxins. [4] GFC is a monophyletic taxon. It causes devastating diseases on economically important plants. Consumption of contaminated food can cause hazardous diseases in humans and animals. It lowers the productivity in livestock and death if prolonged exposure occurs. [4] Gibberella fujikuroi can produce carcinogenic mycotoxins and cause equine leukoencephalomalacia of horses and donkeys. [5] Gibberellins are plant growth hormone. [6] The production of gibberellin by Gibberella fujikuroi is depending on the nature of carbon and nitrogen sources. It is stimulated by high carbon to nitrogen ratio. [7] This fungus is the industrial source of gibberellic acid. 14 strains of Gibberella fujikuroi were screened to select for the most potent strain. [7, 8] Gibberella fujikuroi has affected rice and cause Bakanae disease which is first reported in Japan by Hori in 1989. Bakanae is the condition in which “foolish seedling” which means abnormal excessive elongation of the plant occurs. [9] It is known to be a seed born as well as soil born pathogen of rice and it infect the seedlings during germination. [10] Yabuta and Hayashi (1939) reported that gibberellins causes elongation of other plants, i.e. barley, buckwheat, rapeseed, tomato, cucumber, morning glory etc. [6] Bavistin (Carbendazim) is commonly used fungicide to treat Gibberella fujikuroi fungus of bakanae disease. [10,11] Bavistin may cause genetic defects and damage fertility or the unborn child. EPA has classified Bavistin as Group C: “Possible Human Carcinogen” . [12] Homoeopathy is an art and science of healing where ultra-high dilutions are used as medicine. The principle “Law of minimum dose” means lower the dose higher the effectiveness. Homoeopathy can treat humans as well as plants. [13] Homoeopathic medicines namely Thuja occidentalis, Silicea, Mercurius