Industrial Crops and Products 97 (2017) 49–55 Contents lists available at ScienceDirect Industrial Crops and Products jo ur nal home p age: www.elsevier.com/locate/indcrop Assessment of the terpenic composition of Hedychium coronarium oil from Eastern India Asit Ray a , Biswabhusan Dash a , Ambika Sahoo a , Noohi Nasim a , Pratap Chandra Panda b , Jeetendranath Patnaik c , Biswajit Ghosh d , Sanghamitra Nayak a , Basudeba Kar a,∗ a Centre for Biotechnology, Siksha O Anusandhan University, Bhubaneswar, Odisha, India b Taxonomy and Conservation Division, Regional Plant Resource Centre, Bhubaneswar, Odisha, India c Department of Botany, Sri Krushna Chandra Gajapati College, Paralakhemundi, Odisha, India d Post Graduate Department of Botany, Ramakrishna Mission Vivekananda Centenary College, Kolkata, West Bengal, India a r t i c l e i n f o Article history: Received 6 October 2016 Received in revised form 18 November 2016 Accepted 30 November 2016 Keywords: Essential oil Two dimensional gas chromatography (GCxGC-TOF-MS) Hedychium coronarium J. koenig a b s t r a c t Hedychium coronarium J. koenig (Zingiberaceae) is well known for to its medicinal and aromatic proper- ties because of it terpenic components. The paper aims to characterize the terpenic composition present in the essential oil of Hedychium coronarium J. koenig by two-dimensional gas chromatography with time-of-flight mass spectrometry (GCxGC-TOF-MS). A total of 101 terpenic constituents were identified, 53 of which were reported for the first time. On the basis of their chemical structure, the constituents were grouped into four classes: hydrocarbon monoterpenes, oxygenated monoterpenes, sesquiterpene hydrocarbons and oxygenated sesquiterpenes. The identified compounds include 35 alcohols, 34 hydro- carbons, 12 ketones, 7 aldehydes, 7 oxides, 3 ethers and 3 esters. The predominant compounds identified by GCxGC-TOF-MS were eucalyptol, p-cymene and p-menth-1-en-8-ol. A database containing retention indices of terpenoids was created for the bi-dimensional column, thus proving to be a remarkable step for analysis of terpenoids using a two dimensional gas chromatography (GCxGC) system. GCxGC enhanced the separation efficiency of constituents as well as resolved a number of co-eluting components that were unresolved on a one dimensional gas chromatography column. The current research enabled in compre- hensive profiling of terpenic composition present in H. coronarium oil, an important initiative that could add to the valuation and bioprospecting potential of H. coronarium as a source of terpenic constituents. © 2016 Published by Elsevier B.V. 1. Introduction The Hedychium genus of the family Zingiberaceae comprises around 80 species and is widely distributed in Asia and Madagascar (Joshi et al., 2008). Essential oils extracted from flowers, leaves and rhizomes of these plants possess many medicinal and pharmaco- logical properties (Hartati et al., 2014). Hedychium coronarium J. koenig commonly known as white ginger lily belongs to genus Hedychium. It is widely distributed in Southern China, India, Brazil, and Southeast Asian countries (Morikawa et al., 2002). Rhizomes of this plant are strongly aro- matic and the essential oil obtained from the rhizomes are widely used in perfumery (Matsumoto et al., 1993; Gao et al., 2008). Rhizomes are consumed as stimulant and carminative (Chan and ∗ Corresponding author. E-mail addresses: basu.cbt@gmail.com, basudebakar@soauniversity.ac.in (B. Kar). Wong, 2015). They are also effective in alleviating fever and used as excitant in the Indian Ayurvedic system (Mehta et al., 2014; Parida et al., 2015). Rhizomes are utilized for the medication of diabetes, headache, rheumatism, cancer (Bhandary et al., 1995; Kunnumakkara et al., 2008). Essential oil of H. coronarium possesses significant bioactive properties such as antimicrobial, antioxidant, anti-inflammatory as well as mosquito larvicidal activities (Joy et al., 2007; Sabulal et al., 2007; Joshi et al., 2008; Lu et al., 2009; Ho, 2011). Plant essential oils are usually complex mixtures since they con- tain several hundred of constituents, including alcohols, aldehydes, ketones, esters, oxides and terpenoids (Zhu et al., 2005). Though gas chromatography mass spectrometry (GC–MS) has been used for many years for analysis of volatile constituents, it cannot offer adequate separation of complex compounds because of the poor resolution power of an individual column (Purcaro et al., 2009). Use of a combination of two columns can significantly improve the res- olution to a greater extent. Further, owing to the isomeric nature of sesquiterpene compounds, they co-elute at a similar retention time, http://dx.doi.org/10.1016/j.indcrop.2016.11.063 0926-6690/© 2016 Published by Elsevier B.V.